Effect of Abscisic Acid on NaCI Stressed Callus Proliferation and Plant Regeneration in Rice  

作　　者：ikram-ul-Haq Ghulam Yasin Mumtaz Hussain Ali Mohammad Dahri 

机构地区：[1]Institute of Biotechnology and Genetic Engineering （IBGE）, University of Sindh, Jamshoro 76080, Pakistan [2]Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan [3]Department of Botany, Universuity of Agriculture, Faisalabad 38040, Pakistan

出　　处：《Journal of Life Sciences》2012年第1期48-54,共7页生命科学（英文版）

摘　　要：In rice, 2,4-Dichlorphenoxyacetic acid （MS2） has been considered best for callus induction and combination ofNAA, IAA, ABA （MS3） for somatic embryogenesis. Efficient plant regeneration was observed when MS basal salts supplied with 3% sorbitol and 3% maltose as carbon sources without hormones （MSac）. During this experiment, effect of sodium chloride （NaCl） and abscisic acid （ABA） was assessed on callus growth, plant regeneration and root induction efficiency of rice （Oryza sativa L.） varieties IR-6 and Basmati-370. Callus proliferation rate was highly decreased in both varieties on MS2b （100 mol.m3 NaCl ＋ 0.5 mg＇L1 ABA） than MS2a （100 mol＇m3 NaCl） cultures significantly. Proline, glycine-betaine and reducing sugars were increased significantly in MS2a and MS2b callusing cultures. However, total proteins were decreased in MS2a, while slightly increased in MS2b. Maximum plant regeneration （9.42 ±0.54 and 10.67 ±0.50 plantlets.callus1） from somatic embryos was observed on MS4c in IR-6 and Basmati-370, while 1.56 ± 0.06 （IR-6） and 0.95 ±0.05 （Basmati-370） plantlets callus1 were observed on MSab （100 mol·m3 NaCl）. No plant regeneration was observed on MS4b （100 mol·m3 NaCl ＋ 0.5 mg·L-1 ABA） medium in both varieties. Inhibition of root induction efficiency was high in MSsb （100 mol·m3 NaCl ＋ 0.5 mg·L-1 ABA） than MS5a （100 mol·m3 NaCl） in the stressed cultures （P 〈 0.05）. In this experiment, it was concluded that ABA involved in somatic embryogenesis and elevation of NaCl stress, while it causes inhibition of cell＇s growth as well as its regeneration into plantlets from somatic embryos.

关 键 词：Oryza sativa L. NACL ABA embryogenic callus somatic embryogenesis bio-contents. 

分 类 号：S511.01[农业科学—作物学] Q943.1[生物学—植物学]