糖尿病大鼠颈动脉窦隔离灌注异丙酚对血浆内皮素及一氧化氮水平的影响  

Effect of isolated carotid sinus perfusion with propofol on plasma endothelin and nitric oxide levels in diabetic rats

作　　者：吴卫平[1] 王寿世[1] 孙晓燕[1] 宋婷婷[2] 荆丽君[3] 曹焕军[3] 宁吉顺[1] 

机构地区：[1]青岛大学医学院第二附属医院青岛市中心医院麻醉科,266042 [2]青岛大学医学院第二附属医院青岛市中心医院肿瘤科,266042 [3]潍坊医学院麻醉系

出　　处：《中华麻醉学杂志》2012年第1期48-50,共3页Chinese Journal of Anesthesiology

基　　金：2010年度山东省青岛市科技发展指导计划项目（KZJ-21）

摘　　要：目的探讨糖尿病大鼠颈动脉窦隔离灌注异丙酚对血浆内皮素（ET）及一氧化氮（NO）水平的影响。方法健康清洁级成年雄性Wistar大鼠，体重180～220g，采用腹腔注射链脲佐菌素30mg／kg的方法制备糖尿病模型。取糖尿病模型制备成功的大鼠36只，采用随机数字表法，将其随机分为3组（n=12）：对照组（Dc组）、低浓度异丙酚组（DPI组）和高浓度异丙酚组（DB组）。另取非糖尿病大鼠36只，采用随机数字表法，将其随机分为3组（n=12）：对照组（NC组）、低浓度异丙酚组（NP，组）和高浓度异丙酚组（NP2组）。颈动脉窦隔离灌流K—H液（对照组）、含50μmol／L异丙酚的K—H液（低浓度异丙酚组）和含100ttmol／L异丙酚的K—H液（高浓度异丙酚组），30min后取股动脉血样，测定血浆ET和NO浓度。结果与Dc组比较，DP1组和DP2组血浆ET浓度降低，NO浓度升高（P〈0．05）；与DP1组比较，DP2组血浆ET浓度降低，NO浓度升高，NP1组血浆ET浓度升高，NO浓度降低（P〈0．05）；与DP2组比较，NP1组血浆ET浓度升高，NO浓度降低（P〈0．05）。结论异丙酚可通过对颈动脉窦压力感受器的局部作用降低糖尿病和非糖尿病大鼠血浆ET水平，升高NO水平，且对糖尿病大嗣的枯廊可强。Objective To investigate the effect of isolated carotid sinus perfusion with propofol on plasma endothelin and nitric oxide levels in diabetic rats. Methods Healthy adult Wistar rats weighing 180-220 g were used in this study. Diabetes mellitus was induced by intraperitoneal streptozotocin 30 mg/kg and confirmed by blood glucose ≥ 16.7 mmol/L. Thirty-six rats in which diabetes mellitus model was successfully established were randomly divided into 3 groups （ n = 12） ：control group （group DC） ;low dose propofol group （group DPl ） and high dose propofol group （group DP2 ） . Another 36 non-diabetic rats were also randomly divided into 3 group （n = 12 each） ： control group （ group NC） ; low dose propofol group group （ group NP1 ） and high dose propofol （ group NP2 ） . After 30 min isolated carotic sinus perfusion with K-H solution （control groups）, 50 μmol/L propofol in K-H solution （low dose propofol groups） or 100 μmol/L propofol in K-H solution （high dose propofol groups）, blood samples were taken for determination of plasma endothelin and nitric oxide concentrations. Results The plasma endothelin concentration was lower and nitric oxide concentration higher in groups DPI and DP2 than in group DC, and in group DP1 than in group DP2 （ P 〈 0.05）. The plasma endothelin concentration was higher and nitric oxide concentration lower in group NPI than in group DP1, and in group NP2 than in group DP2 （ P 〈 0.05 ）. Conclusion Propofol can decrease endothelin level and increase nitric oxide level in diabetic or non-diabetic rats through local effect on carotid sinus, and the effect is stronger in diabetic rats than in non-diabetic rats.

关 键 词：二异丙酚 糖尿病 内皮缩血管肽类 一氧化氮 颈动脉窦 

分 类 号：R614[医药卫生—麻醉学] R587.1[医药卫生—外科学]