烤烟ζ-胡萝卜素脱氢酶基因的克隆与生物信息学分析  

Cloning and Sequence Analysis of T-zds1 Gene from Flue-cured Tobacco

作　　者：胡重怡[1] 雷波[1] 刘冬[2] 蔡刘体[1] 

机构地区：[1]贵州省烟草科学研究所,贵阳市金阳新区云潭北路550081 [2]长江大学农学院,湖北省荆州市荆秘路88号434023

出　　处：《烟草科技》2012年第3期34-37,63,共5页Tobacco Science & Technology

基　　金：贵州省烟草专卖局资助项目"烟草类胡萝卜素致香前体物质合成途径关键酶基因克隆及表达研究"(黔烟科2010-12);"贵州省不同风格特色烟叶形成的分子机制研究"(黔烟科2010-02)

摘　　要：利用同源克隆和电子克隆方法,从烤烟栽培品种韭菜坪2号(Nicotiana tobacum cul.Jiucaiping2)中克隆获得烤烟ζ-胡萝卜素脱氢酶(zeta-carotene desaturase,ZDS)基因的全长cDNA序列(命名为T-zds1),并对其编码蛋白的一级、二级进行了预测。该基因在GenBank中的登录号为JF975566,全长为2312 bp,编码的蛋白含588个氨基酸,蛋白登录号AEG73891。BLASTp搜索结果及进化分析结果表明,烤烟T-zds1基因编码氨基酸序列与番茄、向日葵、胡萝卜ZDS基因编码氨基酸序列的相似性分别为97%,92%,90%。Homology cloning and in silico cloning method were used to chlone the full length cDNA sequence of tobacco zeta-carotene dehydrogenase gene（named T-zdsl）from flue-cured tobacco cultivar, Jiucaiping2, and the primary and secondary structures of its encoding protein were predicted. The GenBank accession number of T-zdsl was JF975566 with the full length of 2312 bp, the coding protein contained 588 amino acids and the protein accession number was AEG73891. The results of BLASTp and phylogenetic analysis showed that the similarities of encoding amino acid sequence of T-zdsl to that of tomato, sunflower and carrot were 97%, 92%, and 90%, respectively.

关 键 词：烤烟 基因 克隆 ζ-胡萝卜素脱氢酶 

分 类 号：S572[农业科学—烟草工业]