谷氨酰胺强化的早期肠内营养对严重烫伤大鼠肠黏膜结构及细胞凋亡的影响  

Effects of early enteral nutrition supplement with glutamine on structure and apoptosis of intestinal mucosa in scalded rats

作　　者：赵永华[1] 邱方[1] 孟伟[1] 

机构地区：[1]河北省廊坊市人民医院重症医学科,河北廊坊065000

出　　处：《中国医药导报》2012年第7期22-24,共3页China Medical Herald

摘　　要：目的探讨谷氨酰胺(Gln)对烫伤大鼠肠黏膜结构和细胞凋亡的影响。方法选择健康Wistar大鼠50只随机分为肠内营养组(EN组,20只)、肠内营养加谷氨酰胺补给组(EN+Gln组,20只)和假烫伤组(10只),制备总烧伤面积(TBSA)为25%的Ⅲ度烫伤动物模型,伤后早期分别给予肠内营养制剂能全力和能全力加Gln,于24、72 h观察肠黏膜结构,并检测细胞凋亡和血浆二胺氧化酶活性变化。结果大鼠在严重烫伤后肠黏膜受损,细胞凋亡较假烫伤组增多(P<0.05);与EN组比较,EN+GLn组可使细胞凋亡下调,明显减轻肠黏膜的损伤(P<0.05)。结论 Gln能抑制烫伤大鼠肠黏膜细胞凋亡,保护肠黏膜结构。Objective To investigate the influence of glutamine on structure and apoptosis of intestinal mucosa in scalded rats.Methods 50 healthy adult Wistar rats were randomly divided into enteral nutrition（EN） group（n = 20）,enteral nutrition combined with glutamine（EN＋Gln） group（n = 20） and pseudoscalded（PS） group（n = 10）.Scalded model of Ⅲ degree scald injury（25% total body surface area） was established in the EN and EN＋Gln groups,after injury the two groups were treated with the corresponding methods.The structure of intestinal mucosa was observed after 24 h and 72 h of scald and the chang of DAO activity and apoptosis were detected.Results After scalded the intestinal mucosa of rats were damaged.The apoptosis rates of EN group and EN＋Gln group were higher than that of PS group（P 〈 0.05）.Compared with EN group,EN ＋ Gln group could downregulate the apoptosis,reduce the injury of intestinal mucosa significantly（P 〈 0.05）.Conclusion Gln can inhibit the intestinal mucosa apoptosis of scalded rats and protect the intestinal mucosa structure

关 键 词：谷氨酰胺 烫伤 二胺氧化酶 凋亡指数 

分 类 号：R664[医药卫生—外科学]