Smad4条件基因敲出小鼠的前庭功能检测  

Evaluation of vestibular functions in Smad4 conditional knockout mice

作　　者：邓安春[1] 杨仕明[2] 黄德亮[2] 孙建和[2] 杨晓[3] 

机构地区：[1]第三军医大学新桥医院耳鼻咽喉科,重庆400037 [2]解放军总医院耳鼻咽喉头颈外科,解放军耳鼻咽喉研究所,北京100853 [3]军事医学科学院生物工程研究所发育和疾病遗传学研究室,北京100071

出　　处：《第三军医大学学报》2012年第7期577-580,共4页Journal of Third Military Medical University

基　　金：国家高技术研究发展计划(863计划,2007AA02Z150);国家自然科学基金重点项目(30730040);国家自然科学基金面上项目(3057101730871398);重庆市回国人员启动基金(CSTC2010BB5032);第三军医大学回国人员启动基金(2010XHG05)~~

摘　　要：目的通过设计的一系列前庭功能检测实验对同窝成组的软骨组织Smad4条件基因敲除野生型、杂合子、纯合子小鼠的前庭功能进行评估。方法前庭功能检测方法:一般行为观察;空间姿势反射;游泳实验;前庭眼反射(VOR),使用前庭功能检查系统记录眼震电图;短声刺激前庭诱发肌源性电位(VEMP)记录麻醉下椎前颈长肌表面第3颈椎水平的声刺激诱发电位。结果虽然软骨组织Smad4条件基因敲除小鼠的耳蜗感受声音能力下降,但没有发现软骨组织Smad4条件基因敲除小鼠出现行为、步态、空间姿势反射和游泳实验等前庭综合功能障碍;3个基因型小鼠均能诱出眼震且相互之间没有差别(P>0.05);VEMP的潜伏期在3个基因型组间无差异(P>0.05),与野生型和杂合子相比,纯合子小鼠VEMP的反应阈值明显增加(P<0.01)。结论只有Smad4条件基因敲除纯合子小鼠的部分球囊功能受到一定程度的影响,其他前庭功能不受影响。Objective To evaluate the vestibular functions in Smad4 conditional knockout mice.Methods Vestibular functions were evaluated through general behavioral observation,air righting reflex,and swimming test in 3 genotypes of mice,Smad4＋/＋.Smad4＋/-,and Smad4-/-.Vestibular ocular reflex（VOR） was recorded using vestibular function examination system.Vestibular evoked myogenic potential（VEMP） were recorded by loud clicks evoking on the pre-vertebral muscle at the level of the third cervical vertebral bone using silver ball electrodes under general anesthesia.Results Though cochlear perceiving sound was reduced in Smad4-/-mice,their behaviors,gaits,air righting reflex and swimming test were normal.The 3 genotypes of mice were induced identical nystagmus through rotation,but no difference was found among them（P0.05）.Latency of VEMP had no difference among the 3 genotypes of mice（P0.05）,but the response threshold of VEMP in Smad4-/-mice were obviously elevated（P0.01）.Conclusion Only partial functions of saccule are damaged to some extent in Smad4 conditional knockout mice,and other vestibular endorgans have normal functions.

关 键 词：SMAD4 条件基因敲除 前庭功能 

分 类 号：R322.923[医药卫生—人体解剖和组织胚胎学] R394.3[医药卫生—基础医学]