农药敌磺钠与吖黄素的荧光猝灭反应及其分析应用  

Fluorescence Quenching Reaction of Fenaminosulf with Acriflavine and Their Analytical Applications

作　　者：王亚琼[1] 刘绍璞[1] 刘忠芳[1] 胡小莉[1] 

机构地区：[1]发光与实时分析教育部重点实验室,西南大学化学化工学院,重庆400715

出　　处：《应用化学》2012年第4期440-445,共6页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(20875078);发光与实时分析重庆市重点实验室(CSTC,2006CA8006)资助项目

摘　　要：在pH=5.4的Britton-Robinson(BR)缓冲溶液中,农药敌磺钠(FM)与吖黄素(AF)相互作用,形成离子缔合物,导致吖黄素溶液的荧光猝灭。当分别于最大激发波长和最大发射波长(λex/λem)451 nm/508 nm测量时,荧光猝灭值(ΔF)与敌磺钠浓度在0.16～5.0 mg/L范围内呈良好的线性关系,检出限为0.048 mg/L。据此建立了一种测定敌磺钠的荧光分析新方法。该方法操作简单、灵敏度高、选择性好,用于敌磺钠农药以及土壤中敌磺钠残留检测,结果满意。还研究了反应体系的荧光特性,并结合量子化学AM1计算对荧光猝灭机理进行了讨论。In pH=5.4 Britton-Robinson（BR） buffer solution,fenaminosulf（FM） reacted with acriflavine（AF） to form an ion associated complex,which resulted in the quench of the fluorescences of acriflavine.The maximum excitation wavelength（λex） and emission wavelength（λem） was located at 451 nm and 508 nm.The quenching value（ΔF） was linear to the concentration of FM in the range of 0.16~5.0 mg/L with the detection of limit（3σ） of 0.048 mg/L.Therefore,a simple,rapid,highly sensitive and selective method for the determination of FM by fluorescence quenching had been established.The proposed method had been used to determine FM in fenaminosulf pesticide and soil residue with satisfactory results.In addition,the fluorescence characters of the reaction system and the mechanism of fluorescence quenching were also discussed by AM1 calculations.

关 键 词：敌磺钠 吖黄素 荧光猝灭 

分 类 号：O657.3[理学—分析化学]