五子衍宗丸对无精子症模型小鼠生精能力恢复作用的基因表达谱研究  

Study on Gene Expression Profiling of Wuzi Yanzong Wan in Regaining Spermatogenic Ability in Mouse with Azoospermia

作　　者：魏刚[1] 陈西华[1] 张树成[1] 李云峰[2] 贺斌[1] 王尚明[1] 张斌[1] 王宁[1] 王介东[1] 

机构地区：[1]国家人口计生委科学技术研究所,北京100081 [2]河北医科大学,050091

出　　处：《河北中医药学报》2012年第1期4-7,2,共4页Journal of Hebei Traditional Chinese Medicine and Pharmacology

基　　金：国家自然科学基金:No 90409016;国家重点基础研究发展计划(973计划)项目:No 2010CB530403

摘　　要：目的:研究五子衍宗丸对无精子症模型小鼠生精能力恢复作用的睾丸全基因表达谱。方法:利用白消安无精子症小鼠动物模型,给予五子衍宗丸灌胃给药78天(2个生精周期),以造模后自然恢复、溶媒和正常动物为3种对照,通过基因芯片技术进行数据分析。结果:五子衍宗丸对睾丸生精能力具有明显促进作用,在睾丸全基因表达谱中具有明显调节作用的基因中,差异表达2倍以上的基因842个(占2.65%,上调355个,下调487个),具有较强烈作用的差异表达4倍、5倍以上的上调基因和下调基因分别27个、9个和72个、23个,提示五子衍宗丸在促进生精过程中的基因表达以负向抑制/下调作用为主,以正向促进/上调作用为辅;主要通过对黏附功能、细胞周期、细胞因子-细胞因子受体作用、Hedgehog信号通路、Jak-Stat信号通路、Mapk信号通路、Notch信号通路、Toll样受体信号通路、Wnt信号通路等9个信号通路系统的干预调节实现生精能力提高的效果。结论:五子衍宗丸对无精子小鼠生精能力的恢复作用,主要是对生精干细胞的诱导分化和细胞增殖分裂相关基因的负向下调作用的调节并通过多途径实现的。Objective: to study the testis gene expression profiling of Wuzi Yanzong Wan in regaining spermatogenic ability in mouse with azoospermia.Methods: model mice with azoosperimia induced by busulfan were given intragastric administration with Wuzi Yanzong Wan for 78 days(two spermatoginic cycles),with spontaneous recovery,solvent and normal mice as control before making data analysis with gene chip.Results: Wuzi Yanzong Wan could promote the spermatogenic ability significantly.Among the genes with regulating functions in the gene expression profiling,there were 842 genes with differential expression by twice(acounting 2.65%,up-regulation 355 and down-regulation 487),and 27,9,72 and 23 genes respectively with differential expression by more than 4 or 5 times,which showed that Wuzi Yanzong Wan mainly regulated downward in promoting spermatogenic ability through regulating and interfering in adhesion,cell cycle,cytokine-cytokine receptors,Hedgehog,Jak-Stat,Mapk,Notch,Toll,Wnt signal channels.Conclusion: Wuzi Yanzong Wan can regain the spermatogenic ability of mice with azoospermia by regulating downward genes related to the induction and differentiation of spermatogenic stem cells,and the cell multiplication and division.

关 键 词：五子衍宗丸 基因表达谱 生精干细胞 基因调控 精子质量 

分 类 号：R285.5[医药卫生—中药学]