丙烯酰胺急性染毒对雄性小鼠生殖细胞微管蛋白的影响  

The toxic effect of acrylamide acute exposure on the microtubulin of germ cells in male mice

作　　者：白宝琴[1] 赵倩[1] 黄幸[1] 杨宇君[1] 杨夏[1] 张蓓[1] 杨建一[1] 

机构地区：[1]山西医科大学细胞生物与遗传学教研室,山西太原030001

出　　处：《毒理学杂志》2012年第1期21-24,共4页Journal of Toxicology

基　　金：山西医科大学学生创新项目基金资助项目(2009年)

摘　　要：目的研究丙烯酰胺(AA)急性染毒对雄性小鼠生殖细胞微管蛋白的影响。方法 36只健康雄性小鼠,随机分为阴性对照组,15、25和50 mg/kg剂量组,每组9只,腹腔注射连续染毒6 d,间接免疫荧光技术测睾丸细胞微管蛋白的表达量。结果不同剂量AA染毒后小鼠体重增加,睾丸与体重的比值差异无统计学意义(F=0.878,P=0.466)。生殖细胞微管蛋白与对照组相比,15 mg/kg组差异无统计学意义(P>0.05),25 mg/kg组和50 mg/kg组差异具有统计学意义(P<0.05)。结论在本实验条件下,AA染毒对小鼠生殖细胞微管蛋白产生毒性影响,且存在剂量效应关系。Objective To study the toxic effect of acute acrylamide exposure on the microtubulin of germ cells in male mice.Methods Thirty-six healthy male mice were randomly divided into four groups,including negative control,15 mg/kg,25 mg/kg and 45 mg/kg treatment groups.Each group contains nine mice.Acrylamide was administrabyed intraperitoneally（ip） to the mice of treatment groups for 6 successive days.The microtubulin content of germ cells were detected using indirect immunofluorescence technique.Results The body weight increased in treatment groups.The ratio of testis and weight was no significant difference（F=0.878,P=0.466）.Compared with the control group,there were significant difference of the microtubulin of germ cells in the 25 mg/kg and 40 mg/kg group（P〈0.05）,but there was no difference in 15 mg/kg group（P〉0.05）.Conclusion Acrylamide exposure can induce toxic effect on the microtubulin of germ cells in male mice,and shows a dose-dependent relationship.

关 键 词：丙烯酰胺 生殖毒性 微管蛋白 

分 类 号：R994.6[医药卫生—毒理学]