乙型肝炎病毒感染者外周血CD4＋T细胞CD25-CD127-表达的检测及临床意义  

Clinical significance and detection of the expression of CD25 - CD127 - on CD4 ＋ T cells in peripheral blood in patients with hepatitis B

作　　者：叶军[1] 陈亚宝[1] 徐洪涛[1] 成建春[1] 张立新[1] 林梅[1] 黄俊星[1] 

机构地区：[1]江苏省泰州市人民医院临床医学研究所,泰州225300

出　　处：《中华实验和临床病毒学杂志》2011年第6期474-476,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的 检测乙型肝炎病毒（ HBV）感染者外周血CD4＋T细胞表面CD25 - CD127 -的表达情况及临床意义.方法 用流式细胞术比较分析53例慢性乙肝患者、53例HBV携带者和26例正常对照人群CD4＋T细胞表面CD25 - CD127 -的表达情况,并对20例用干扰素治疗的HBV-DNA阳性慢性乙肝患者随访.结果 ①与正常对照组比较,慢性乙肝患者、HBV携带者CD4＋ CD25 -CD127 -T细胞均显著升高,两者比较有统计学差异（Q =4.559,P＜0.05；Q=6.230,P＜0.05）；②HBV- DNA阳性患者（n=77） CD4＋ CD25 - CD127 -T细胞显著低于HBV- DNA阴性患者（n=29）,两者比较有统计学意义（t =2.290,P=0.024）；③与治疗前比,慢性乙肝患者干扰素治疗12周后CD4＋CD25- CD127 -T细胞显著降低,两者比较差异有统计学意义（t =2.469,P=0.024）.结论 乙型肝炎病毒感染者外周血CD4＋ CD25 - CD127 -T细胞与病毒的感染和清除相关,外源性干扰素可降低CD4＋ CD25 - CD127 -T细胞.Objective To investigate the clinical significance and detection of the expression of CD25 - CD127 - on CD4 ＋ T cells in peripheral blood in patients with hepatitis B.Methods The expression of CD25- CD127- on CD4 ＋ T cells were measured by using flow cytometry in 53 patients with chronic hepatitis B,53 carrier with hepatitis B virus and 26 healthy blood donors,and follow up 20 patients with HBV-DNA positive treated with interferon.Results ① Compared with healthy controls,the expression of CD25 - CD127 - on CD4 ＋ T cells in patients and carrier with hepatitis B virus were lower（ Q =4.559,P 〈0.05 ; Q =6.230,P 〈 0.05 ）.②The expression of CD25 - CD127 - on CD4 ＋ T cells in patients with HBV-DNA positive（ n =77 ） was lower than that of negative （ n =29 ） （ t =2.290,P =0.024 ）.③Compared with the prior treatment,the expression of CD25 - CD127 - on CD4 ＋ T cells in patients with B hepatitis were lower after interferon treated with 12 weeks（t =2.469,P =0.024）.Conclusion It suggested that the CD25-CD127- expression on CD4 ＋ T cells correlated with viral infections and cleared,exogenous interferon could decrease CD25 - CD127 - expression on CD4 ＋ T cells.

关 键 词：肝炎病毒 乙型 T淋巴细胞 受体 白细胞介素2 受体 白细胞介素7 流式细胞术 

分 类 号：R51[医药卫生—内科学]