黄连解毒汤水溶液体系化学成分与树脂吸附-筛分原理关联的初步研究  

Preliminary Study on Relevance between Chemical Components in Hunaglian Jiedu Decoction and Adsorption and Screen Separation Theory of Macroporous Resin

作　　者：朱华旭[1] 邱碧菡[1] 李欢[1] 郭立玮[1] 潘林梅[1] 

机构地区：[1]南京中医药大学中药复方分离工程重点实验室,南京210029

出　　处：《中国实验方剂学杂志》2012年第7期1-5,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30873450);江苏省教育厅‘青蓝工程’项目(2008)

摘　　要：目的:探讨中药复方水溶液体系下黄连解毒汤中主要药效成分与树脂吸附和筛分原理之间的内在关联,为黄连解毒汤的剂型改进奠定实验基础。方法:选择目前市售的11种不同类型大孔树脂进行静态吸附和解析实验。RP-HPLC比较各组水溶液体系精制前后生物碱、黄酮、环烯醚萜苷三大类药效中指标性成分含量的动态变化。结果:不同类型大孔树脂对黄连解毒汤中三大类药效成分的吸附率、解析率差异均较大。极性较小、平均孔径较大的X-5,AB-8型树脂对生物碱类的吸附率、解析率均较高;表面积和平均孔径较大的X-5,ADS-17,ADS-7,NKA-9,D151型树脂对黄酮类成分的富集作用较好;平均孔径较大的X-5,S-8,NKA-Ⅱ型树脂和极性较大的ADS-7,D151型树脂对环烯醚萜苷类成分的富集作用较好高。结论:在中药复方水溶液体系下,采用树脂组合的方式,可以优化目标产物的分离工艺。Objective：To investigate inherent relevance between primary effective ingredients in Hunaglian Jiedu decoction and theory of adsorption and screen separation about macroporous resins under aqueous solution system of Chinese herbal compound,and to lay experimental basis for improvement of dosage forms about Hunaglian Jiedu decoction.Method： 11 type of commercially available macroporous resins were selected to carry out static adsorption and desorption experiments.The dynamic contents change of three categories of index ingredients including alkaloids,flavonoids and iridoid glycoside were compared before and after purification by RP-HPLC.Result： Both adsorption and desorption rates of three categories of primary effective ingredients in Huanglian Jiedu decoction by different macroporous resins had remarkable differences.X-5 and AB-8 resin had both higher adsorption and desorption rates for alkaloids with weaker polarity and bigger particle size;X-5,ADS-17,NKA-9 and D151 resin had a better enrichment effect for flavonoids with bigger surface area and pore size;X-5,S-8 and NKA-Ⅱ resin had better enrichment effect for iridoid glycoside with bigger average pore size,while ADS-7 and D151 resin had the same effect with stronger polarity.Conclusion： In aqueous solution system of Chinese medicine compound,using resin combination could optimize separation process of target products.

关 键 词：黄连解毒汤 不同极性大孔树脂 三类药效成分 吸附-解析动力学 

分 类 号：R283.6[医药卫生—中药学]