星点设计-效应面法优化天山雪莲传递体制备处方  

Optimization of Preparation Formulation of Saussurea involucrata Transfersomes by Central Composite Design/Response Surface Methodology

作　　者：马晓莉[1] 邢建国 王新春[1,3] 樊鑫梅[1] 连文元[1] 谢敏[1] 

机构地区：[1]石河子大学药学院,新疆石河子832002 [2]新疆维吾尔自治区药物研究所,乌鲁木齐830004 [3]石河子大学医学院一附院新疆石河子832008

出　　处：《中国实验方剂学杂志》2012年第7期5-8,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30960528)

摘　　要：目的:优化天山雪莲传递体的处方。方法:注入-高剪切分散法制备天山雪莲传递体,以绿原酸和芦丁包封率、载药量、传递体平均粒径及归一化值为评价指标,以磷脂浓度、药脂比(质量比)、油水相体积比为考察因素,星点设计-效应面法优化处方,实验结果进行多元线性和二项式非线性方程拟合,效应面法确定最佳处方配比。结果:二项式是描述各指标与因素之间的最佳模型。以绿原酸为指标的包封率83.44%,载药量4.65%;以芦丁为指标的包封率70.69%,载药量3.94%,载药传递体平均粒径154.6 nm,理论预测值与实测值偏差较小,模型具有良好的预测性。结论:星点设计-效应面优化法筛选天山雪莲传递体是一种简便、可行的实验设计方法。Objective：To optimize formulation of Saussurea involucrate transfersomes.Method： S.involucrata transfersomes was prepared by combining injection technique with high shear scattered method,effects of three independent variables including the concentration of phospholipid,ratio of drug-lipid and volume ratio of oil phase-water phase were investigated,with entrapment efficiency and drug loading of chlorogenic acid and rutin,mean particle size and normalized value as evaluation indexes,central composite design/response surface method was used to optimized formulation,test results were fitted by multiple linear and binomial nonlinear equations,and optimum formulation was selected by response surface method.Result： Binomial was optimum model to describe between indexes and factors.According to optimal preparation conditions of S.involucrate transfersomes,entrapment efficiency of chlorogenic acid and rutin were 83.44%,70.69 respectively,drug loading were 4.65%,3.94% respectively,and average particle size with 154.6 nm,there was small deviations between theoretical value and measured value,this model had good predictability.Conclusion： Central composite design/response surface method was a feasible and convenient method to optimize prescription of S.involucrate transfersomes.

关 键 词：星点设计-效应面法 优化处方 天山雪莲 传递体 星点设计-效应面优化法 制备 平均粒径 实验结果 

分 类 号：R283.6[医药卫生—中药学]