HPLC测定四季三黄片中5种蒽醌类衍生物的含量  

Five Anthraquinone Derivatives in Siji Sanghuang Tablets Determined by HPLC

作　　者：冯源[1] 张德全[1] 周浓[1] 姜北[1] 康小丽[1] 

机构地区：[1]大理学院,云南大理671000

出　　处：《中国实验方剂学杂志》2012年第7期96-99,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立四季三黄片中同时测定芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚含量的方法。方法:色谱柱为Agela Venusil XBP-C18柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%磷酸,梯度洗脱,流速1.0 mL.min-1,检测波长226nm。结果:芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚分别在0.040～0.800,0.040～0.800,0.040～0.800,0.040～0.800,0.020～0.400μg呈良好的线性关系,平均回收率(n=6)分别为97.62%,99.48%,99.72%,96.23%,95.68%,RSD1.62%,1.24%,1.13%,1.19%,1.67%。结论:所建立的定量方法快速简单,方法可靠,可作为四季三黄片的质量控制方法。Objective：To develop a method for simultaneous determining aloe emodin,rhein,emodin,chrysophanol and physcion in Siji Sanghuang tablets.Method： The method was conducted on Agela Venusil XBP-C18（4.6 mm×250 mm,5 μm） column.The mobile phase was acetonitrile（A） and 0.1% phosphoric acid（B） with gradient elution.The flow rate was 1.0 mL·min-1 and the detection wavelength was 226 nm.Result： The linear ranges of aloe-emodin,rhein,emodin,chrysophanol and physcione were 0.040-0.800,0.040-0.800,0.040-0.800,0.040-0.800,0.020-0.400 μg respectively,the average recoveries（n=6） were 97.62%（RSD 1.62%）,99.48%（RSD 1.24%）,99.72%（RSD 1.13%）,96.23%（RSD 1.19%）,95.68%（RSD 1.67%） respectively.Conclusion： The method is accurate,reliable,repeatable and can be used for the quality control of Siji Sanghuang tablets.

关 键 词：四季三黄片 高效液相色谱法 蒽醌 含量测定 

分 类 号：R284.1[医药卫生—中药学]