HPLC-DAD-MS^n联用技术表征大川芎方效应组分中主要化学成分  

Characterization on Central Ingredients of Active Components in Dachuanxiong Fang based on HPLC-DAD-MS^n

作　　者：沈岚[1,2] 林晓[1,2] 梁爽[1] 洪燕龙[1] 袁莹[1] 冯怡[1,2] 徐德生[3] 阮克锋[1] 

机构地区：[1]上海中医药大学中药现代制剂技术教育部工程研究中心,上海201203 [2]上海中医药大学中药学院,上海201203 [3]上海中医药大学附属曙光医院,上海200021

出　　处：《中国实验方剂学杂志》2012年第7期128-134,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金青年科学基金项目(30801548);上海市教育委员会科研创新项目(12ZZ124;11zz111);上海市自然基金(11ZR1434500);上海市教委重点学科资助(J50302)

摘　　要：目的:采用HPLC-DAD-MSn联用技术研究大川芎方效应组分的主要化学成分,为其体内物质基础研究及质量标准的完善奠定基础。方法:通过建立大川芎方效应组分指纹图谱,将其复杂的化学成分表征出来,并采用HPLC-DAD-MSn联用技术对各化学成分进行结构鉴定和推测。结果:推断出川芎效应组分中的3个化学成分和天麻效应组分中的8个化学成分。结论:大川芎方效应组分的化学成分研究有助于阐明其效应物质基础并完善其质量标准。Objective：To investigate central ingredients of active components in Dachuanxiong fang（DF） by HPLC-DAD-MSn,so that the quality of DF as well as its preparations can be effectively and systemically controlled and the substance basis of DF can be explored.Method： HPLC fingerprint spectrum of the active parts of Dachuanxiong fang was established.HPLC-DAD-MSn was developed for identification of the active parts of Dachuanxiong fang.Result： Three components of the active parts of chuanxiong and 8 components of the active parts of tianma were identified.Conclusion： Research on central ingredients of active components in Dachuanxiong fang is favorable to the clarification of its active substance basis and perfection of quality stantard.

关 键 词：大川芎方 效应组分 液质联用 化学成分 

分 类 号：R284.1[医药卫生—中药学]