不同批次枳壳中柚皮苷、新橙皮苷、总黄酮、挥发油的含量比较及质量评价  

Comparison of Content of Naringin,Neohesperidin,Total Flavonoid and Volatile Oil in Different Batches of Citrus aurantium and Quality Evaluation

作　　者：王元清[1,2] 严建业[1] 师白梅[1] 李顺祥[1] 罗堃[1] 王璐[1] 黄海卫 

机构地区：[1]湖南中医药大学药学院中药现代化教育厅重点实验室,长沙410208 [2]中南林业科技大学生命科学与技术学院生物技术与工程实验室,长沙410004 [3]长沙市第三医院药剂科,长沙410007

出　　处：《中国实验方剂学杂志》2012年第7期146-150,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖南省科技厅项目(2009TP4051-1);长沙市科技计划项目(k1003034-31);湖南省高校“中药新药创制与资源综合持续利用”科技创新团队项目(湘教通[2010]212号);国家中医药管理局“药用植物学”重点学科项目;湖南省“中药学”重点学科项目

摘　　要：目的:比较不同批次枳壳中柚皮苷、新橙皮苷、总黄酮、挥发油的含量,并评价其质量。方法:高效液相色谱法,色谱柱为Diamonsil C18(4.6 mm×250 mm,5μm),流动相乙腈-水(20∶80)(磷酸调pH至3.0);检测波长283 nm,流速1 mL.min-1,柱温30℃,测定柚皮苷和新橙皮苷;以柚皮苷为对照,采用紫外-可见分光光度法在283 nm处测定总黄酮;采用水蒸气蒸馏法提取测定挥发油含量;以柚皮苷含量、新橙皮苷含量、总黄酮含量、挥发油含量为综合评价指标,采用Topsis法评价其质量。结果:柚皮苷含量范围在0%～6.12%,新橙皮苷含量在0%～4.55%,总黄酮含量在6.96%～18.99%,挥发油量在0.2%～2.0%(mL.g-1);17个批次样品质量差异较大。结论:17批次枳壳中柚皮苷、新橙皮苷、总黄酮、挥发油的含量有较大差异;柚皮苷、新橙皮苷含量达到2010年版《中国药典》标准的仅6批,质量评价方法可行。Objective：To compare the content of naringin,neohesperidin,total flavonoids and volatile oil in different batches of Citrus aurantium and evaluate the quality.Method： The naringin and neohesperidin were detected by HPLC.The analytical column was a Diamonsil C18（4.6 mm×250 mm,5 μm）.A mixture of acetonitrile-water（pH was adjusted to 3.0 by phosphoric acid）（20∶80） was used as the mobile phase with the flow rate at 1 mL·min-1.The detection wavelength was set at 283 nm.The column temperature was set at 30 ℃.The UV spectrophotometry for the determination of total flavonoids is as follows： detection wavelength was at 283 nm,and the reference substance was naringin.The volatile oil was extracted by steam distillation.Topsis was employed for evaluating quality with the content of naringin,neohesperidin,total flavonoids and volatile oil.Result： The naringin,neohesperidin,total flavonoids and volatile oil were in the ranges of 0%-6.12%,0%-4.55%,6.96%-18.99% and 0.2%-2.0%（mL·g-1） respectively.The quality difference of the samples was large.Conclusion： The content difference of the naringin,neohesperidin,total flavonoids and volatile oil in 17 different batches of C.aurantium was large.The content of naringin and neohesperidin in 6 batches of C.aurantium comes up with 〈Chinese Pharmacopoeia 2010〉.The quality evaluation method was feasible.

关 键 词：枳壳 柚皮苷 新橙皮苷 总黄酮 挥发油 质量评价 

分 类 号：R284.1[医药卫生—中药学]