射干提取物含药血清对豚鼠离体气管平滑肌收缩功能的影响  

Effects of Serum Containing Belamcanda chinensis Extract on the Contraction of Isolated Guinea-pig Tracheal Smooth Muscle

作　　者：甘雨[1] 乔敏[1] 张宏[1] 邹德俊[2] 李国信[1] 

机构地区：[1]辽宁省中医药研究院,沈阳110034 [2]辽宁中医药大学,沈阳110032

出　　处：《中国实验方剂学杂志》2012年第7期164-166,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部"十一五"重大新药创制项目(2009ZX09103-330)

摘　　要：目的:研究射干提取物含药血清对豚鼠离体气管平滑肌收缩功能的影响。方法:取豚鼠气管,在Kreb’s液中制备气管平滑肌螺旋条,于恒温浴槽装置中,加入组胺(His)使其收缩,观察加入射干提取物含药血清后3,5,10 min的解痉率。结果:射干提取物含药血清对组胺引起的气管平滑肌收缩反应有一定拮抗作用,其中剂量(0.46 g.kg-1)3,5,10 min解痉率分别为44.29%,45.27%和48.30%;高剂量(0.92 g.kg-1)3,5 min解痉率分别为31.17%和31.83%,与空白对照组比较有显著性差异(P<0.05或P<0.01),药物与标本接触5 min效果最好。结论:射干提取物含药血清对His引起的气管平滑肌收缩反应具有一定的拮抗作用。Objective：To investigate the effects of Belamcanda chinensis extract on the contraction of isolated guinea-pig tracheal smooth muscle by serum pharmacological method.Method： The guinea-pig tracheal smooth muscle was isolated in the Kreb＇s solution,and its contraction was induced by histamine（His）.The inhibitory effects of serum-containing drug on contraction of isolated tracheal strips were observed,and the constriction rate was calculated in 3,5,10 minute.Result： The serum-containing drug could significantly inhibit the contraction of the guinea-pig tracheal smooth muscle induced by His,in the dose 0.46 g·kg-1 group in 3,5,10 min antispasmodic rate was 44.29%,45.27%,48.30%;high dose（0.92 g·kg-1） in 3,5 min antispasmodic rate was 31.17% and 31.83%,compared with the control group there was a significant difference（P0.05 or P0.01）,and the effects were better in 5 minute.Conclusion： The B.Chinensis extract has inhibitory effect on contraction of tracheal smooth muscle induced by His.

关 键 词：射干提取物 血清药理学 气管平滑肌 

分 类 号：R285.5[医药卫生—中药学]