慢性乙肝患者外周血单个核细胞中CXCR1、CXCR2及IL-8 mRNA表达与干扰素治疗关系  

Influence of IFN on expression of chemokine receptor CXCR1,CXCR2 and their ligand IL-8 in the patients with chronic hepatitis B

作　　者：毕惠娟[1] 王健[1] 黄河胜[2] 刘军华[1] 

机构地区：[1]安徽理工大学医学院病原学与免疫学教研室,安徽淮南232001 [2]安徽医科大学出版中心,安徽合肥230000

出　　处：《细胞与分子免疫学杂志》2012年第4期422-425,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：安徽省自然科学基金资助项目(090413138);安徽省教育厅自然基金重点项目(KJ2009A032)

摘　　要：目的:了解乙型肝炎病毒(HBV)慢性感染患者外周血单个核细胞(PBMC)中CXCR1、CXCR2及IL-8 mRNA表达水平及与α干扰素(IFN-α)治疗的关系。方法:采用实时定量PCR法动态观察30例慢性乙型肝炎患者接受IFN-α治疗前、治疗3个月、6个月后其外周血单个核细胞CXCR1、CXCR2及IL-8 mRNA表达水平。结果:治疗前慢性乙肝患者CXCR1、CXCR2及IL-8 mRNA表达水平分别为(0.44740.0386)、(0.4720 0.0458)、(1.1897 0.1028),均高于正常对照组(n=36),其中CXCR1及IL-8 mRNA水平升高显著,差异有统计学意义(P<0.01)。治疗过程中CXCR1、CXCR2及IL-8表达水平均显著下降。IFN-α治疗6个月后CXCR1、CXCR2及IL-8 mRNA表达水平分别为(0.41290.0395)、(0.4461 0.0477)、(0.8660 0.1307),与治疗前相比,差异有显著性(P<0.01或P<0.05)。治疗前的CX-CR1、CXCR2及IL-8的表达水平在HBV高复制组(HBV-DNA>106,n=22)明显高于HBV低复制组(HBV-DNA<106,n=8),差异有统计学意义(P<0.05)。结论:慢性乙肝患者外周血单个核细胞中CXCR1和IL-8表达水平显著升高,在干扰素治疗后,其表达水平下调,证明其可能与慢性乙肝炎症的发生机制相关。AIM： To study the mRNA levels of chemokine receptor CXCR1, CXCR2 and IL-8 in the patients with chronic hepatitis B and their association with IFN therapy. METHODS： The mRNA levels of CXCR1, CXCR2 and IL-8 in peripheral blood mononuclear cells （PBMC）of chronic hepatitis B patients were determined by real-time RT-PCR before and during the IFN-α therapy. RESULTS： The mRNA levels of CXCR1 （0.4474 ± 0.0386） and IL-8 （ 1. 1897±0.1028） in peripheral blood of the patients with chronic hepatitis B were significantly higher than those in healthy donors（P〈0.01）. The mRNA level of CXCR2（0.4720 ± 0.0458） was higher than those in healthy donors, but there was no significant differences between the two groups. During the treatment, the mRNA levels of CXCR1, CXCR2 and IL-8 obviously decreased. After treatment for six month, the mRNA level of CXCR1 （0.4129 ± 0.0395 ）, CXCR2 （0.4461±0.0477） and IL-8（0.8660±0.1307）were significantly lower than those before treatment（ P 〈 0. 01 or P 〈 0.05）. Additionally, the expressive levels of CXCR1, CXCR2 and IL-8 in the high HBV loading group （ HBV-DNA 〉 10^6 ） were much higher than those in the low HBV loading group （HBV-DNA〈10^6）. CONCLUSION： CXCR1 and IL-8 may contribute to hepatic inflammation. Among them, CXCR1, CXCR2 and IL-8 decrease after IFN treatment, which illustrates that IFN-α plays an important role in down-regulating inflammation response, controlling the development of the patients＇ condition.

关 键 词：慢性乙型肝炎 趋化因子受体 CXCR1 CXCR2 IL-8 干扰素-Α 

分 类 号：R392.12[医药卫生—免疫学]