呼吸道合胞病毒毛细支气管炎患儿外周血CD4^+CD25^+调节性T细胞与Th17细胞功能变化及意义  

Changes and the clinical significance of CD4^+CD25^+ regulatory T cells and Th17 cells in peripheral blood of infants with respiratory syncytial virus bronchiolitis

作　　者：李宾[1] 吴福玲[2] 冯学斌[1] 孙大康[3] 崔晴晴[1] 赵志旭[2] 

机构地区：[1]滨州医学院儿科学教研室,山东滨州256603 [2]滨州医学院附属医院儿科,山东滨州256603 [3]滨州医学院临床医学实验中心,山东滨州256603

出　　处：《细胞与分子免疫学杂志》2012年第4期426-428,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：山东省自然科学基金资助项目(ZR2009CL007)

摘　　要：目的:探讨呼吸道合胞病毒(RSV)毛细支气管炎患儿外周血CD4+CD25+调节性T细胞和Th17细胞及其分泌细胞因子IL-10、TGF-β、IL-17水平变化与RSV毛细支气管炎发病的关系。方法:收集2010-09/2011-04在滨州医学院附属医院儿科住院的33例RSV毛细支气管炎患儿、28例做为阳性对照的非RSV感染性肺炎患儿(肺炎组)及26例正常对照组的健康体检儿外周血,采用流式细胞术(FCM)检测外周血CD4+CD25+调节性T细胞、Th17细胞百分率,酶联免疫吸附(ELISA)法检测血浆IL-10、TGF-β、IL-17的水平。结果:RSV毛细支气管炎患儿外周血CD4+CD25+调节性T细胞、IL-10、TGF-β水平显著低于肺炎患儿及健康体检儿(P<0.05),而Th17、IL-17水平则显著高于肺炎患儿与健康体检儿(P<0.05)。结论:RSV毛细支气管炎患儿外周血存在CD4+CD25+调节性T细胞与Th17细胞表达失衡,可能是RSV毛细支气管炎发病机制之一。AIM： To observe the percentages of CD4^＋ CD25^＋ regulatory T cells （Tregs） and Th17 cells and the levels of IL-10, TGF-β and IL-17 in peripheral blood of infants with respiratory syncytial virus （RSV） bronchiolitis. The relationship between above cells, cytokines and RSV bronchiolitis was determined. METHODS： Thirty-three infants with RSV bronchiolitis, twenty-eight infants with nonRSV pneumonia and twenty-six healthy infants were enrolled. The percentages of Tregs and Th17 cells in peripheral blood were detected by flow cytometer （FCM）, and the levels of IL-10, TGF-β and IL-17 in plasma were determined by ELISA. RESULTS： The percentage of Tregs and the levels of IL-10 and TGF-β in infants with RSV bronchiolitis were significantly lower than those in infants with non-RSV pneumonia and healthy infants （ P 〈 0. 05 ）, while the percentage of Th17 cells and the level of IL-17 in infants with RSV bronchiolitis were significantly higher than those in infants with non-RSV pneumonia and healthy infants （ P 〈 0. 05 ）. CONCLUSION： The imbalance between Tregs and Th17 cells in peripheral blood of infants with RSV bronchiolitis may be one of the pathogenesis of RSV bronchiolitis.

关 键 词：毛细支气管炎 调节性T细胞 TH17 RSV 儿童 

分 类 号：R725.6[医药卫生—儿科]