反相液相色谱中测定死时间的新方法  

A New Method for the Measurement of Dead Time in Reversed Phase High Performance Liquid Chromatography

作　　者：张博[1] 张庆合[1] 单亦初[1] 张维冰[1] 张玉奎[1] 

机构地区：[1]中国科学院大连化学物理研究所

出　　处：《现代科学仪器》2000年第2期26-27,共2页Modern Scientific Instruments

摘　　要：本文根据同系物保留值的碳数规律 ,提出了一种测定反相液相色谱死时间的新方法 ,并以烷基苯同系物为例 ,加以实验验证。结果表明 ,以这种方法测得的死时间准确可靠 ,并且与正相色谱中的情况类似 ,反相液相色谱中死时间标记物与固定相之间也存在着相互作用。A new method for the measurement of dead time in Reversed Phase High Performance Liquid Chromatography (RPHPLC) was investigated.The method bases on the charcoal_number rule of homologous.Eight alkyl_benzene series were tested,and the data was simultaneously calculated.The calculated value of dead time has the same trend changing with the experimented value, but a little shorter each.The results show the new method is feasible and interaction between mobile phase and stationary phase in RPHPLC is negligible.

关 键 词：反相液相色谱 保留值方程 死时间 测定 

分 类 号：O657.7[理学—分析化学]