亲水性材料固相萃取-紫外导数光谱法检测血中茚满二酮类抗凝血杀鼠剂  

Determination of Indendione Anticoagulant Rodenticides in Blood by Solid phase Extraction on Hydrophilic Material and UV Derivative Spectrophotometry

作　　者：刘冬娴[1] 吴国平[1] 林岱 谭家镒[1] 

机构地区：[1]中国刑警学院法医系,沈阳市皇姑区塔湾街83号110035

出　　处：《光谱实验室》2000年第1期47-49,50,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：公安部科研基金

摘　　要：本文报导了血中茚满二酮类抗凝血杀鼠剂敌鼠、氯敌鼠、杀鼠酮的亲水性材料固相萃取－紫外导数光谱检测法。血样用ｐＨ５ 缓冲液等体积稀释后加于西雷脱硅藻土柱上，柱用乙酸乙酯洗脱，将洗脱液挥发干净，剩余物用 ｐＨ１０ 缓冲液溶解，测定所得溶液的二阶导数光谱进行定性和定量分析。三种杀鼠剂以１０ｍ ｇ／Ｌ的量加于空白血中测得萃取率为８５％ 以上，血中三种杀鼠剂的检出限低于２ｍ ｇ／Ｌ。萃取中亲水性硅藻土可用硅胶和氧化铝代替，结果相似。The determination of indendione anticoagulant rodenticides,such as diphacinone,chlorophacinone and pindone,in blood samples by solid phase extraction on Celite and subsequent UV derivative spectrophotometry is described.Blood samples are diluted with equivalent volume of pH5 buffer and are then poured over columns of Celite.The columns are eluted with ethyl acetate and then the elutes are evaporated to dryness.The residues are dissolved with pH10 buffer.The second derivative spectra of the solutions are measured and used for qualitative and quantitative analysis.The extraction efficiencies of three rodenticides spiked in blank blood samples at a level of 10mg/L are above 85%,and the detection limits of the three rodenticides in blood are below 2mg/L.In extraction procedure the diatomaceous earth can be replaced by silane or alumina and similar results are obtained.

关 键 词：杀鼠剂 血液 紫外导数光谱法 茚满二酮 

分 类 号：D919.2[医药卫生—法医学] TQ456.3[政治法律—法学]