白杨杂种三倍体小孢子母细胞减数分裂及花粉形态观察  

Meiosis of Microsporocyte and Variation of Pollen in an Allotriploid White Poplar

作　　者：许重阳[1] 王君[2] 叶粟[1] 康向阳[1] 

机构地区：[1]北京林业大学林木育种国家工程实验室,北京100083 [2]林木花卉遗传育种教育部重点实验室,北京100083

出　　处：《西北植物学报》2011年第12期2454-2458,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：北京林业大学大学生创新性实验计划

摘　　要：通过醋酸洋红压片等方法观察毛新杨×银腺杨杂种三倍体小孢子母细胞减数分裂及花粉形态,以探讨杂种三倍体雄配子的育性及其细胞学机制。结果显示:(1)在小孢子母细胞减数分裂过程中可观察到部分染色体提前分向两极、落后染色体、微核等一系列染色体异常行为,表明该三倍体存在染色体的高度不平衡性。(2)在减数第二次分裂过程中存在大量胞质分裂提前现象;减数分裂产物中发现有少量二分体、三分体的存在,同时毛新杨×银腺杨杂种三倍体花粉粒的直径变化范围为22.6～62.6μm,呈现双峰分布,由此推测有一定数量的具可育性的未减数花粉产生。Abstract：Variation of pollen and meiosis ot mtcrosporocyte in an allotriploid white poplar [（Populus to- mentosa× P. bolleana） × （P. alba ×P. glandulosa ） ] were investigated by the squashed technique. Numer- ous meiotic abnormalities, including precocious migration of chromosomes to the poles, lagging chromo- somes,and micronuclei were found, which indicated highly genetic imbalance of this allotriploid. A great number of precocious cytokinesis was found during the second meiotic division. There were dyads and triads in meiotic products,and the diameters of the pollen grains of the allotriploid white poplar ranged from 22. 6 to 62.6μm,with a bimodal frequency distribution. That indicated the production of some unreduced pol- len grains,which were fertile.

关 键 词：毛新杨×银腺杨 杂种三倍体 小孢子母细胞减数分裂 可育性花粉 

分 类 号：Q944.58[生物学—植物学]