痕量糖-8-氨基芘-1,3,6-三磺酸衍生物的毛细管电泳-激光诱导荧光分析  

Analysis of Saccharides by Capillary Electrophoresis with Laser-induced Fluorescence Detection

作　　者：党福全[1] 陈义[1] 郭晴[1] 

机构地区：[1]中国科学院化学研究所分子科学中心,北京100080

出　　处：《分析化学》2000年第1期80-83,共4页Chinese Journal of Analytical Chemistry

基　　金：国家杰出青年基金!29825112;中国科学院重大基金!KJ951-Al-507

摘　　要：以芘为原料经磺化、氨化合成了8-氨基芘-1，3，6-三磺酸钠盐（APTS），建立了相应的纯化方法，考察了多种条件对还原单糖衍生效率的影响，研究单糖及葡聚糖水解寡糖-APTS。衍生物的毛细管电泳-激光诱导荧光分离与检测，单糖的检测限在amol（10^(18))水平。A novel carbohydrate labeling agent of 8-aminopyrene-1,3,6-trisultonate (APTS) has been synthesized from pyrene in two steps and purified in our laboratory. Using monosugars and dextran as testing Sample, labeing and capillny electrophorsis separation conditions have been investigated. The detection sensitivity can easily reach attomole level when using a home-made laser-induced fluorescence detection system.

关 键 词：APTS 寡糖链 毛细管电泳 激光诱导荧光 

分 类 号：O657.8[理学—分析化学]