缺氧缺血新生鼠脑损伤后银杏叶对Nogo-A蛋白表达的影响  

Influence of ginkgo biloba extract on neural stem cell Nogo-A protein expression after cerebral hypoxia ischemic in newborn rats

作　　者：宋文秀[1] 范勇波[1] 吴晓媚[2] 

机构地区：[1]遵义医学院第五附属医院儿科,广东珠海519100 [2]遵义医学院第五附属医院病理科,广东珠海519100

出　　处：《贵州医药》2011年第10期880-882,共3页Guizhou Medical Journal

摘　　要：目的探讨银杏叶对新生大鼠缺氧缺血性脑损伤后Nogo-A表达的影响。方法72只7d龄SD大鼠随机分为银杏叶治疗组、对照组和假手术组,应用免疫组化的方法观察银杏叶对脑组织Nogo-A表达的影响。结果脑组织Nogo-A的表达在对照组和银杏叶治疗组3d开始增高,21d达到最高峰,对照组增高幅度最大,其平均光密度值与假手术组和银杏叶治疗组比较差异有显著性(P<0.05)。结论Nogo-A在HIBD后表达增高,可能抑制了中枢神经系统的再生;银杏叶治疗后可减少脑组织Nogo-A的表达,使其对神经细胞再生的抑制作用减弱。Objective To study the effects of ginkgo biloba extract on expression of Nogo-A in the neonatal rat brain tissue after hypoxia ischemic brain damage （HIBD）. Methods 72 neonatal SD rats were divided randomly into three groups which were ginkgo biloba extract group , controlled group and sham-operated group. Immunohistochemistry method was used to measure the expressions of No- go-A after in the neonatal rat brain tissue after hypoxia ischemic brain damage. ResultsThe expression of Nogo-A in the neonatal rat brain tissue in ginkgo biloba extract group and controlled group increased at 3 d after hypoxia ischemic brain damage and reached the highest level at 21 day. The corrected optb cal density（COD） value of controlled group was significantly higher than that of the sham-operation group and ginkgo biloba extract group（P〈0. 05）. Conclusion Ginkgo biloba extract can reduce （he ex- pression of Nogo-A protein in the brain and thus promote neuronal regeneration in neonatal rats fol- lowinu I-HIBD

关 键 词：NOGO-A 银杏叶提取物 缺氧缺血性脑损伤 

分 类 号：R971[医药卫生—药品]