甘草酸二铵对慢性乙型肝炎患者外周血CD4^+CD25^+Foxp3^+调节性T细胞的影响  

Effect of diammonium glycyrrhizinate on CD4^+CD25^+Foxp3^+ regulatory T cells in patients with chronic hepatitis B

作　　者：周建康[1] 

机构地区：[1]绍兴市第六人民医院肝病科,312000

出　　处：《中华危重症医学杂志（电子版）》2011年第6期9-12,共4页Chinese Journal of Critical Care Medicine:Electronic Edition

摘　　要：目的探讨应用甘草酸二铵后,慢性乙型肝炎(CHB)患者外周血CD4+CD25+Foxp3+调节性T细胞(Treg)的变化情况。方法选取CHB患者60例(CHB组),甘草酸二铵治疗30d并采集治疗开始前、开始后15d、治疗结束后第2天的外周血,同时选择60例乙肝表面抗原阴性的健康体检者作为对照组。采用三色荧光标记流式细胞术检测两组外周血中CD4+CD25+Foxp3+Treg的含量。结果 CHB组在治疗开始前外周血Treg高于对照组(P<0.05);治疗15d后,外周血Treg含量较治疗开始前下降,但仍高于对照组(P均<0.05);治疗30d后Treg含量进一步下降,与前两次相比,差异均具有统计学意义,且仍高于对照组(P均<0.05)。结论外周血Treg可能参与CHB患者乙型肝炎病毒(HBV)免疫耐受。甘草酸二铵可降低患者外周血Treg数量,提示其可能通过对HBV的免疫调节实现其治疗作用。Objective To evaluate the effect of diammonium glycyrrbizinate on the CD4＋ CD25＋Foxp3＋ regulatory T cell （Treg） in peripheral blood of chronic hepatitis B （CHB） patients. Methods Diammonium glycyrrhizinate were given to 60 patients with CHB by intravenous drip for 30 d and peripheral blood samples were collected before treatment, on 15th d after treatment began, and 2nd d after treatment ended. Sixty healthy people with hepatitis B surface antigen negative were served as controls and randomly collected peripheral blood samples. The levels of Treg in the two groups were analyzed by tricolor fluorescence labeled flow cytometry. Results The levels of Treg in the CHB group before treatment were higher than the controls, significantly decreased on 15th d after treatment began, and further declined after treatment ended （all P 〈 0.05）. The levels of Treg on 2nd d after treatment ended were much lower than 15th d after treatment began, but both still higher than the controls （all P 〈 0.05）. Conclusions Treg might participate in immune tolerance of hepatitis B virus （HBV） in patients with CHB. Diammonium glycyrrhizinate could decrease the level of Treg, induce HBV immunoregulation, and thereby achieve curative effect.

关 键 词：肝炎 乙型 慢性 甘草次酸 调节性T细胞 流式细胞术 

分 类 号：R512.62[医药卫生—内科学]