寡糖-8-氨基芘-1,3,6-三磺酸衍生物的毛细管电泳-激光诱导荧光分离  

Ultrasensitive Fluorometric Detection of Oligosaccharides as Their APTS Derivatives by Capillary Electrophoresis

作　　者：党福全[1] 陈义[1] 郭晴[1] 徐桂芸[1] 

机构地区：[1]中国科学院化学研究所分子科学中心,北京100080

出　　处：《高等学校化学学报》2000年第2期206-209,共4页Chemical Journal of Chinese Universities

基　　金：国家杰出青年基金!(批准号 :29825112);中国科学院重大基金!(批准号:KJ951-A1-507)资助

摘　　要：利用自组装的毛细管电泳 -激光诱导荧光装置 ,研究了多种寡糖 -8-氨基芘 -1 ,3 ,6-三磺酸 (寡糖 -APTS)衍生物的分离 .考察了电泳介质、浓度及 p H对寡糖 -APTS衍生物分离的影响 ,在酸性和碱性条件下 。Reducing oligosaccharides, after reductive amination, were separated and detected as their APTS derivatives by capillary electrophoresis with laser-induced fluorescence. Brief optimization studies have also been performed with respect to CE separation conditions of oligosaccharides. The oligosaccharide ladders were analyzed in an acidic phosphate-tris buffer(pH 25) or in the alkaline borate buffer. Acid hydrolysis products of dextran T-40 labeled with APTS were also successfully mapped.

关 键 词：寡糖 葡聚糖 毛细管电泳 激光诱导荧光 分离 

分 类 号：Q533[生物学—生物化学] O658.9[理学—分析化学]