鸡痘病毒282E_4株基因组3.6kbBamHI片段序列测定与分析  

Sequence Analysis of a 3.6kb BamHI Genomic Fragment of Fowlpox Virus Strain 282E 4

作　　者：罗坤[1] 金宁一[1] 郭志儒[1] 王风华 吴丛梅 顾万均 殷震[1] 

机构地区：[1]解放军军需大学军事兽医研究所

出　　处：《中国预防兽医学报》2000年第1期34-38,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家863 计划!(批准号:101_05_03_1) 资助项目

摘　　要：本实验将我国FPV282E4 株基因组3 .6kb BamHID片段利用DNA测序仪进行了序列测定。经DNASISV3.0 分析,该片段A+ T含量为60.77 % ,内含有8 个主要的ORFS。用Goldkey V3.0 软件比较了VVP7.5 早期启动子,FPV4b 晚期启动子、L2R早/晚期启动子和一个早/ 晚期双向启动子与各个ORF上游100bp 区域的同源性,没有发现有意义的同源序列;该片段的核苷酸序列和每个ORF所编码的氨基酸序列在EMBL核酸序列库和蛋白质序列库(Swiss_PROT) 中没有找到有意义的同源序列。The nucleotide sequence of BamHI genomic fragment from fowlpox virus(FPV)strain 282E 4 has been determined by ABI PRISM TM 377 DNA sequencer.Analysis of the sequence revealed 8 potential major open frames(ORF S)by the use of DNASIS V3.0. The homology was compared between four early/late promoters of FPV/VV and the region of 100bp immediately upstream of each ORF with the help of Goldkey V3.0. We searched for the homologue of the amino acid sequences that each ORF coded within Swiss_PROT protein datebase. We speculated this fragment was a non_essential or a non_coding region in FPV genome.

关 键 词：鸡痘 病毒 基因组片段 序列测定 282E4株 

分 类 号：S852.65[农业科学—基础兽医学] Q78[农业科学—兽医学]