风信子DNA不同提取方法的效果比较  

Comparison of Genomic DNA Extraction Methods From Hyacinth

作　　者：胡凤荣[1,2] 任翠[2] 鲍仁蕾[2] 罗凤霞[3] 

机构地区：[1]北京林业大学,北京100083 [2]南京林业大学,南京210037 [3]金陵科技学院,南京210038

出　　处：《沈阳农业大学学报》2011年第5期570-573,共4页Journal of Shenyang Agricultural University

基　　金：国家林业局"948"项目(2009-4-12);中国博士后科学基金面上项目(20100470217);江苏省高校优势学科建设工程资助项目

摘　　要：采用简易CTAB法、改良CTAB法、SDS-CTAB法、高盐法和CTAB-硅珠法等5种方法对风信子花蕾、叶片和鳞片基因组DNA进行提取。比较DNA纯度、电泳、得率等指标,结果表明:CTAB-硅珠法没有获得DNA,其他方法均可提出DNA;在纯度方面,简易CTAB法>SDS-CTAB法>改良CTAB法>高盐法;在得率方面,简易CTAB法>改良CTAB法>高盐法>SDS-CTAB法。简易CTAB法提取的DNA纯度较高,OD260/OD280为2.01,OD260/OD230为2.33,浓度为406.8ng.μL-1,得率为175.95ng.μL-1。花蕾、叶片适合风信子基因组DNA的提取。Five methods including simple CTAB,CTAB improved method,SDS-CTAB method,high salt precipitation method and silica-purification method were used to extract genomic DNA from different parts of Hyacinth such as bud,leaves,scale.The results were compared by three parts,purity,the electrophoreses results and yield of the extracted DNA.The results showed that all of these DNA extraction methods except for CTAB-silica-purification method could obtain DNA.The purity of extracted DNA was as following ： simple CTAB method SDS-CTAB method CTAB improved methodhigh salt precipitation method,and yield as following ： simple CTAB methodCTAB improved methodhigh salt precipitation methodSDS-CTAB method.Simple CTAB method could obtain more purity DNA than the other methods and OD260/OD280 was 2.01,OD260/OD230 was 2.33.The concentration was 406.8ng·L-1 and the yield was 175.95ng·g-1.The extraction results from different parts showed that flower buds and leaves were both suit for Hyacinth obtain DNA.

关 键 词：风信子 基因组DNA 提取方法 

分 类 号：S682.2[农业科学—观赏园艺]