基于Special-Base模型检测转基因作物nos/plamid构建特异性  

Special-Base GMCs detection using multiple PCR and pyrosequencing

作　　者：兰青阔[1] 王永[1] 赵新[1] 陈锐[1] 朱珠[1] 李欧静[1] 余景会[1] 郭永泽[1] 程奕[1] 

机构地区：[1]天津市农业科学院中心实验室/农业部转基因产品成分监督检验测试中心,天津300381

出　　处：《中国农业大学学报》2012年第2期8-12,共5页Journal of China Agricultural University

基　　金：科技部国际合作项目(2006DFA32380)

摘　　要：以转基因作物中常见的nos/plamid构建特异性序列为研究对象,通过同源性比较和焦磷酸测序dispensation order设计,建立Special-Base焦磷酸测序检测模型;利用转基因大豆Roundup Ready Soybean和转基因玉米Bt11、GA21、NK603等4种转基因作物为试验材料,针对nos/plamid构建特异性序列设计引物,经复合PCR扩增及焦磷酸测序,验证Special-Base焦磷酸测序检测模型有效性。结果表明:本研究建立的Special-Base焦磷酸测序模型能检测出样品中单一或多种转基因作物成分,提高了转基因作物检测的精确性、检测通量和自动化程度。Despite the various technologies in place for GMC test,a robostic method is highly demanded. The technology described here represents a breakthrough development in that direction. Aimming to nos/plamid gene of four GMCs （RRS,Bt11, GA21, NK603）, by combining the multiple PCR with pyrosequencing, we developed a rapid assay for genotyping nos/plamid that relies on the identification of a single, type-specific ＇sentinel＇ base. The prototype assay was developed to recognize the four GMCs and was capable of recognizing simultaneously multiple GMCs infections. The studies established a rapid, reliable and cost-effective assay.

关 键 词：转基因作物(GMC) 复合PCR 焦磷酸测序 Special-Base nos/plamid 

分 类 号：S338[农业科学—作物遗传育种]