UV-B辐射处理小麦苗期叶片的蛋白质组分析  

Analysis of wheat seeding leaf proteome after UV-B radiation

作　　者：黄修文[1] 张艳菊[1] 冯清[1] 陈伟霞[1] 张文会[2] 李保云[1] 

机构地区：[1]中国农业大学农学与生物技术学院/北京市作物遗传改良重点实验室/农业部作物基因组学与遗传改良重点开放实验室/教育部作物杂种优势研究与利用重点实验室,北京100193 [2]聊城大学生命科学学院,山东聊城252059

出　　处：《中国农业大学学报》2012年第2期31-36,共6页Journal of China Agricultural University

基　　金：国家自然科学基金资助项目(31071412);国家"973"计划项目(2009CB118300)

摘　　要：本研究以小麦品种聊大1号为试验材料,研究UV-B辐射对聊大1号苗期叶片蛋白质组的影响。双向电泳分析发现,UV-B辐射处理和未处理的聊大1号有15个蛋白差异点,经质谱分析成功鉴定出6个蛋白差异点:其中1个为Rubisco大亚基(碳水化合物代谢);2个为参与翻译类的蛋白;2个为具有运输类功能的蛋白;1个为质子-ATP酶α亚基。这些结果可为小麦抗UV-B辐射机理研究和抗性育种提供依据。In order to investigate the results of UV- In order to investigate the results of UV-B radiation on the accumulation of proteins in wheat seeding leaves,the cultivar LiaoDa 1 was subjected to UV-B radiation stress.Compared with control samples,the UV-B radiation stressed samples accumulated significantly higher amounts of protein.These proteins were separated by two-dimensional electrophoresis（2-DE）,visualized by silver staining and analyzed by Image Master.It revealed 15 differentially expressed polypeptides,6 of which were successful identified by MALDI-TOF,including a Rubisco large subunit,an ATP synthase CF1 alpha chain,proteins involved in protein translation and transportation.These results might provide information for further UV-B radiation stress study in wheat.

关 键 词：小麦 苗期 UV-B辐射 蛋白质组 双向电泳 

分 类 号：S512[农业科学—作物学]