南沙参中多糖含量测定方法的建立及比较  

Establishment and Comparison of Content Determination Methods for Polysaccharides in Radix Adenophorae

作　　者：冯泽岸[1,2] 巩慧敏[1,2] 邵婷玑[1,2] 廖明琪[1,2] 梁莉[1] 乔华[1] 王婷[1] 

机构地区：[1]兰州大学第一医院药物临床试验机构,兰州730000 [2]兰州大学药学院

出　　处：《中国药师》2012年第3期290-293,共4页China Pharmacist

基　　金：2007年甘肃省中医药管理局科研项目(甘卫中发[2007]123号)

摘　　要：目的:建立南沙参中多糖含量的测定方法并进行比较。方法:采用水提醇沉法分离提取南沙参多糖,以葡萄糖为对照制备标准曲线;采用蒽酮-硫酸法和苯酚-硫酸法,利用紫外分光光度计测定多糖含量。结果:蒽酮-硫酸法测定南沙参中多糖含量为16.37%,加样回收率为101.1%,RSD为2.1%;苯酚-硫酸法测定南沙参中多糖含量为15.26%,加样回收率为99.9%,RSD为1.8%。结论:两种方法操作简单,精密度高、重复性好,均可用于南沙参中多糖含量的测定。Objective：To establish and compare the methods for the content determination of polysaccharides in Radix Adenophorae. Method：The polysaccharides were isolated and extracted from Radix Adenophorae by water extraction and alcohol precipitation. The standard curve of polysaccharides from Radix Adenophorae was obtained with glucose as the control.The content of polysaccharides was determined by anthrone sulphuric acid colorimetry and phenol-vitriolic colorimetry using a UV spectrophotometer.Result：The content determined by anthrone-sulphuric acid colorimetry was 16.37%with average recovery of 101.1%（RSD =2.1%）,and that determined by phenol-vitriolic colorimetry was 15.26%with average recovery of 99.9%（RSD = 1.8%）.Conclusion：Both methods for the content determination of polysaccharides in Radix Adenophorae are reliable and accurate,and can be considered as the assay methods for polysaccharides in Adenophora tetraphylla（thunb.） Fisch.

关 键 词：南沙参 南沙参多糖 蒽酮-硫酸法 苯酚-硫酸法 含量测定 

分 类 号：R927.2[医药卫生—药学]