Determination of lisinopril using anion exchange chromatography with integrated pulsed amperometric detection  

作　　者：Yu Xiu Liu Dan Shou Mei Lan Chen Zhi Dong Chen Pei Min Zhang Yan Zhu 

机构地区：[1]School of Chemistry and Chemical Engineering,Changzhou University,Changzhou 213164,China [2]Zhe Jiang Academy of Traditional Chinese Medicine,Hangzhou 310007,China [3]College of Biology and Environment Engineering,Zhejiang Shuren University,Hangzhou 310015,China [4]Department of Chemistry,Xixi Campus,Zhejiang University,Hangzhou 310028,China

出　　处：《Chinese Chemical Letters》2012年第3期335-338,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos20775070,and 20911140271);the Natural Science Foundation of Zhejiang Province(NoR4080124)

摘　　要：A rapid and practical method for direct detection of lisinopril in anion exchange chromatography（AEC） has been developed with integrated pulsed amperometric detection（IPAD）.Dionex AS 18（250 mm×2 mm） and AG 18（50 mm×2 mm） columns and 40 mmol/L NaOH solution were used for separation.Multi-step potential waveform parameters were optimized to maximize the signal-to-noise ratio（S/N）.Utilizing the optimized waveform,the repeatability（intra-day） precision and intermediate（inter-day） precision were obtained with relative standard deviation（RSD） of 0.74,0.93,respectively.The limit of quantification（LOQ） and limit of detection（LOD） were found to be 0.37,0.12ng/mL,respectively,with the correlation coefficient of 0.9998 over concentration range 0.01-1μg/mL.The present method was successfully applied to the determination of lisinopril in human plasma.The recoveries of plasma sample spiked by 0.2μg/mL,0.8μg/mL lisinopril were 98.31-103.23%with RSD of 1.41%, 0.61%,respectively.A rapid and practical method for direct detection of lisinopril in anion exchange chromatography（AEC） has been developed with integrated pulsed amperometric detection（IPAD）.Dionex AS 18（250 mm×2 mm） and AG 18（50 mm×2 mm） columns and 40 mmol/L NaOH solution were used for separation.Multi-step potential waveform parameters were optimized to maximize the signal-to-noise ratio（S/N）.Utilizing the optimized waveform,the repeatability（intra-day） precision and intermediate（inter-day） precision were obtained with relative standard deviation（RSD） of 0.74,0.93,respectively.The limit of quantification（LOQ） and limit of detection（LOD） were found to be 0.37,0.12ng/mL,respectively,with the correlation coefficient of 0.9998 over concentration range 0.01-1μg/mL.The present method was successfully applied to the determination of lisinopril in human plasma.The recoveries of plasma sample spiked by 0.2μg/mL,0.8μg/mL lisinopril were 98.31-103.23%with RSD of 1.41%, 0.61%,respectively.

关 键 词：LISINOPRIL Integrated pulsed amperometry Anion exchange chromatography Angiotensin converting enzyme inhibitor PLASMA 

分 类 号：TF816[冶金工程—有色金属冶金] TH832[机械工程—仪器科学与技术]