子痫前期和子痫孕妇胎盘血小板源性生长因子-B的表达及意义  

Expression and significance of platelet.derived growth factor-B in the placentas from pregnant women with pre-eclampsia or edampsia

作　　者：葛榕[1] 杨茵[1] 林丽珍[1] 方圆[1] 

机构地区：[1]福建医科大学省立临床学院福建省立医院妇产科,福州350001

出　　处：《中国医师进修杂志》2012年第9期1-4,共4页Chinese Journal of Postgraduates of Medicine

基　　金：福建省卫生厅青年科研课题（2009-2-1）

摘　　要：目的通过比较正常妊娠、子痈前期和子痫孕妇胎盘组织血小板源性生长因子（PDGF）．BmRNA与PDGF-BB蛋白的表达，探讨PDGF-B与子痫前期、子痫发病的关系。方法在20例轻度子瘸前期（轻度子痫前期组）、20例重度子痫前期（重度子痫前期组）、3例子痫（子痫组）和20例正常妊娠（对照组）孕妇分娩后取胎盘母体面绒毛组织，采用RT-PCR法检测PDGF-BmRNA表达情况，采用免疫组织化学法结合计算机显微图像分析系统检测PDGF-BB蛋白表达情况。结果轻度子痫前期组、重度子痫前期组和子痫组胎盘组织PDGF-BmRNA和PDGF-BB蛋白表达量（1．56±0．30和1．32±0．34、1．91±0．28和2．00±0A1、2．57±0．06和2．73±0．15）明显高于对照组（1．38±0．25和1．01±0．26），差异有统计学意义（P〈0．01），重度子痫前期组和子痫组明显高于轻度子痫前期组，差异有统计学意义（P〈0．01）。相关分析表明，PDGF．BmRNA和PDGF-BB蛋白表达量均与疾病的严重程度呈正相关（r=0．691、0．797，P〈0．01）。结论子痫前期孕妇胎盘组织高度表达PDGF-B，其可促进动脉粥样硬化形成，可能在子痫前期孕妇的胎盘血管病变中起重要作用，并参与子痫前期的发生和发展。Objective To investigate the relationship between platelet-derived growth factor （PDGF）-B and pre-eelampsia or eclampsia by comparing the expression of PDGF-B mRNA and PDGF-BB protein in the placentas from pregnant women with normal pregnancy, pre-eclampsia or eclampsia. MethodsThe expression of PDGF-B mRNA and PDGF-BB protein were respectively determined with RT-PCR and immunohistochemistry combined with the analysis of computer microphotography in the placentas from 20 mild pre-eclampsia （mild pre-eclampsia group）, 20 severe pre-edampsia （severe pre-eclampsia group）, 3eclampsia （ eclampsia group） and 20 normal pregnancy （control group）. Results The expression of PDGF-B mRNA and PDGF-BB protein in the placentas of mild pre-eclampsia group, severe pre-eclampsia group andeclampsia group （ 1.56 ± 0.30 and 1.32 ± 0.34, 1.91 ± 0.28 and 2.00 ± 0.41, 2.57 ± 0.06 and 2.73 ± 0.15）were higher than those of control group （ 1.38 ± 0.25 and 1.01 ± 0.26） with significant differences （P〈 0.01 ）. The expression of PDGF-B mRNA and PDGF-BB protein in the placentas of severe pre-eclampsia group and eclampsia group were higher than those of mild pre-eclampsia group （P 〈 0.01 ）. The expression of PDGF-BmRNA and PDGF-BB protein were positively correlated with the degree of the disease （r = 0.691,0.797,P 〈0.01 ）. Conclusions Pregnant women with pre-eclampsia and eclampsia have higher expression of PDGF-B in the placentas. PDGF-B may play an important role in the development and progression of pre-eelampsia and eelampsia by promoting atherogenesis in the placental vessels.

关 键 词：血小板源性生长因子 先兆子痫 子痫 动脉粥样硬化 

分 类 号：R714.244[医药卫生—妇产科学]