锰对PC12细胞的增殖抑制与凋亡研究  

Oxidative Stress Mechanism of Manganese-treated PC12 Cell Line

作　　者：徐强 董大海 缪珊[2] 李金翠 高琨[4] 高丽莉[4] 侯顺利[4] 左晶[4] 刘红[4] 闫文[4] 杨银书[4] 卢娟[4] 徐文[4] 

机构地区：[1]兰州市中医医院,甘肃兰州730020 [2]中国人民解放军第四军医大学药物研究所,陕西西安710032 [3]兰州军区第一医院,甘肃兰州730050 [4]兰州军区疾病预防控制中心,甘肃兰州730020

出　　处：《现代生物医学进展》2012年第5期836-839,共4页Progress in Modern Biomedicine

基　　金：甘肃省自然科学基金课题(1010RJZA058)

摘　　要：目的:研究锰作用下PC12细胞的增殖抑制作用与凋亡相关的形态学、生化指标改变。方法:用200,400,600,800μmol/LMnCl2的培养液,分别作用对数生长期PC12细胞1,2,3,4d后,用MTT筛选锰的细胞毒性剂量;透射电镜观察细胞形态学变化;琼脂糖凝胶电泳检测MnCl2对PC12细胞基因组DNA的影响。结果:MTT实验显示200-800μmol/L MnCl2作用4天对PC12有显著的抑制作用,呈剂量和时间依赖趋势,600μmol/L MnCl2作用4d对PC12的抑制率可达50%以上。600μmol/L MnCl2作用4d电镜可见细胞凋亡,同样条件下细胞DNA碎片化。结论:PC12细胞在锰作用下发生增殖抑制,原因是锰诱导PC12细胞凋亡。Objective： To investigate the apoptosis effect of Mn on the PC 12 cell line by detecting the cell morphology and biochemical changes. Methods： PC12 cells in logarithm period incubated in medim with 200, 400, 600, 800pumol/L manganese（MnC12） for 1 day,2 days, 3 days,4days respectively; The cell viability was detected by MTT [3-（4,5- Dimethylthiazol-2-yl）-2,5-diphenyl tetrasoliu- mBromide]; Morphological changes of PC12 cells was investigated by transmisssion electron microscope; Agarose gel electrophoresis was used to detect the genomic DNA of Mn-treated PC 12 transmisssion electron microscope as well as biochemical hallmark of DNA fragments. Results： The results of MTT revealed that manganese of different Concentrations （MnC12200,400,600,800umol/L） could suppress the proliferation of PC12 cells in dose and time-dependent manner. The cell inhibited ratio at the fourth day in 600umol/L MnC12 culture medium approached 50% or more. In the same condition apoptosis was observed in cells. Conclusion： Mn has generated apoptosis which induced proliferation arrested of PC 12 cells.

关 键 词：锰 鼠嗜铬神经瘤细胞(PC12) 氧化应激 凋亡 

分 类 号：Q95-3[生物学—动物学] Q593.1