腹腔注射酵母多糖后小鼠脾脏Foxp3的变化  

Changes of Foxp3 in Splenocytes of Mouse after the Abdominal Injection of Zymosan

作　　者：曹丽[1] 杨双文[1] 贾文元[1] 王世全[1] 王丛丛[1] 李丽[1] 何二涛[1] 汪晨[1] 

机构地区：[1]第四军医大学西京医院麻醉科,陕西西安710032

出　　处：《现代生物医学进展》2012年第5期846-848,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30972854)

摘　　要：目的:通过观察小鼠症状和脾脏大小及脾脏中Foxp3的变化,阐述脾脏Foxp3在酵母多糖(zymosan)所致炎性反应中的作用。方法:采用免疫荧光染色检测正常和腹腔注射酵母多糖后各个时间点小鼠脾脏中Foxp3的变化过程。结果:小鼠6h出现中毒症状,1d,2d时症状最重,死亡率最高;Foxp3在脾脏中的表达在6h时开始减少,3d最少,5d时开始增加(P<0.05)。结论:小鼠脾脏Foxp3变化过程为先下降后升高;Treg在酵母多糖引起的炎症反应中起着重要的作用。Objective： To investigate the effect of Foxp3 on inflammatory response induced by Zymosan by study the changes of mouse symptoms and the changes of Foxp3 in splenocyres. Methods： Immtmofluorescence and Real-time PCR are used to investigate the expression of Foxp3 in spleen. Results： The poisoning symptoms occured at 6h, the symptoms became the heaviest at ld and 2d with the highest mortality, Foxp3 in spleen started to reduce at 6h, reaching the least at 3d, and it began to increase at 5d. Conclusions： The change process of Foxp3 decreased firstly and then increased. This study indicated that Treg may play an important role in zymosan induced inflammatory response.

关 键 词：酵母多糖 SIRS 调节性T细胞 转录因子FOXP3 

分 类 号：R392.12[医药卫生—免疫学]