复方中药制剂在寒冷诱导机体损伤中作用及其机制  

The Effect and Mechanism of Traditional Chinese Medicine Compounds on Cold Induced Organism Injury

作　　者：吕辉[1] 张文斌[1] 孟姗姗[1] 李丹[1] 张建彬[1] 骆文静[1] 刘明朝[1] 陈景元[1] 陈耀明[1] 

机构地区：[1]第四军医大学军事预防医学系军队劳动与环境卫生学教研室,陕西西安710032

出　　处：《现代生物医学进展》2012年第5期856-860,855,共6页Progress in Modern Biomedicine

摘　　要：目的:研究寒冷所诱导的机体损伤作用,明确寒冷引发机体损伤的部分机制以及预防性药物对机体处于寒冷环境中的保护作用。方法:通过低温试验、ATP检测、电镜观察等确定寒冷对机体造成的损伤;通过总抗氧化能力、CAT含量、谷胱甘肽过氧化物酶含量、SOD水平、MDA水平等的检测确定氧化应激在寒冷暴露对机体损伤中的作用及机制;并确证复方中药制剂在寒冷损伤氧化应激状态下对机体的保护作用。结果:1.急性-15±1℃寒冷环境暴露可引起机体内肝脏组织能量代谢障碍,ATP生成减少,肝细胞线粒体损伤,溶酶体增多等机体损伤的现象;2.寒冷应激可导致机体的总抗氧化能力、CAT显著减少,而氧化应激终产物之一的丙二醛(malondialdehyde,MDA)显著增多(说明肝脏组织出现明显的氧化应激过程),并且出现抗氧化能力下降。3.预先给予药物干预后,机体抗氧化能力显著增加。结论:1.寒冷可以诱导机体肝脏明显损伤。2.氧化应激是寒冷诱导机体损伤的关键机制之一。3.复方中药制剂可以增加机体在寒冷应激条件下的抗氧化能力,从而产生保护作用。Objective： To investigate the effect of cold induced body injury, identify the mechanism of cold induced body injury and clear protection effect of traditional Chinese medicine compounds on body in cold environment, provide experimental basis on cold injury and pre-prevention. Methods： The damage caused by the cold on the body was detected by low-temperature test, ATP test, electron microscopy; The effect and mechanism of oxidative stress on body under cold stress was detected by total antioxidant capacity, CAT content, content of glutathione peroxidase, SOD levels and MDA levels; The protective effect of traditional Chinese medicine compounds in cold injury and oxidative stress were also confirmed. Results： 1. Acute exposure to -15＋ 1 degrees cold environment can cause liver energy metabolism, ATP formation decrease, mitochondrial damage in liver cells, and the lysosomal level increase; 2. Cold stress can lead a decreasing of body total antioxidant capacity and CAT level and a increasing of MDA level. 3. Pre-administration of traditional Chinese medicine compounds can enhance the body total antioxidant capacity. Conclusion： 1. Cold can induce significant liver damage 2. Oxidative stress is a key mechanism in cold injury 3. Traditional Chinese medicine compounds can enhance the body total antioxidant capacity and lead a protect effect in cold stress.

关 键 词：寒冷应激 SD大鼠:线粒体损伤 氧化应激 

分 类 号：Q95-3[生物学—动物学] R122[医药卫生—环境卫生学]