香烟烟雾暴露对大鼠肺组织Wnt3a和β-catenin蛋白表达的影响  

Effects of exposure to cigarette smoke on expression of Wnt3a and β-catenin protein in rat lung tissue

作　　者：邹威凤[1] 胡国平[1] 李冰[2] 冉丕鑫[1] 

机构地区：[1]广州医学院第一附属医院广州呼吸疾病研究所呼吸疾病国家重点实验室,510182 [2]广州医学院实验医学研究中心

出　　处：《中华生物医学工程杂志》2012年第1期6-10,共5页Chinese Journal of Biomedical Engineering

摘　　要：目的探讨香烟烟雾暴露对大鼠肺组织Wnt3a和β-catenin蛋白表达的影响。方法20只雌性SD大鼠，按随机数字表分为对照组和模型组，每组10只。模型组大鼠被动吸入香烟烟雾，每天2次，每次45min；对照组大鼠不接触香烟烟雾。100d后处死动物，光镜下观察肺组织的病理形态学改变，免疫组化法检测肺组织α-平滑肌肌动蛋白（α-SMA）、Wnt3a和β-catenin蛋白表达，并测定2组大鼠支气管平滑肌厚度。结果模型组大鼠肺组织呈现慢性炎性反应和肺气肿样改变，小气道壁及平滑肌层增厚明显。α-SMA主要表达于气道和血管的平滑肌层。模型组支气管平滑肌厚度高于对照组[（3．06±0．62）μm比（1．86±0．43）μm，P〈0．05]。Wnt3a和β-catenin主要表达于支气管上皮细胞和肺泡上皮细胞。模型组与对照组支气管上皮细胞Wnt3a蛋白表达分别为74．54±4．14、89．24±3．02；肺泡上皮细胞Wnt3a蛋白表达分别为91．97±2．50、110．46±3．85，组间比较差异均有统计学意义（均P〈0．05）。模型组与对照组支气管上皮细胞胞质β-catenin蛋白表达分别为86．97±4．87、103．18±3．77；支气管上皮细胞胞核β-catenin蛋白表达分别为95．75±3．91、116．12±5．76；肺泡上皮细胞胞质β-catenin蛋白表达分别为106．24±4．57、128．81±3．96；肺泡上皮细胞胞核β—catenin蛋白表达分别为125．44±4．89、152．90±4．10，组间比较差异均有统计学意义（均P〈0．05）。结论香烟烟雾可诱导大鼠肺组织Wnt3a和β-catenin蛋白表达匕调。Objective To investigate the effects of cigarette smoke（CS） exposure on expression of Wnt3a and β-catenin protein in rat lung tissue. Methods Twenty female SD rats were allocated to 2 groups （n= 10 each） by random digit table： the control group and CS exposure group （model group）. Rats in model group were subjected to twice-daily 45-min CS exposure, while the rats in control group were kept isolated from CS. The animals were sacrificed on day 100 to be harvested for lung tissues which were then studied for pathological morphology under light microscopy, expressions of or-smooth muscle actin（ot-SMA）, Wnt3a and β-catenin proteins by immunohistochemistry, and thickness of bronchial smooth muscle layer by morphometry. Results The rats in model group showed chronic inflammation, emphysema-like change and thickening of small airway walls and smooth muscle layers in the lung tissues. In both groups, ct-SMA protein was localized in the smooth muscle layer of airway and vessel. The mean thickness of bronchial smooth muscle was significantly greater in the model group than those in control group [ （3.06±0.62） μm vs （1.86±0.43）μm, P〈0.05]. Wnt3a and β-catenin were mainly expressed in the bronchial and alveolar epithelium. In model group and control group, the levels of Wnt3a expression were 74.54 ± 4.14 vs 89.24 ± 3.02 in bronchial epithelial cells, and 91.97 ± 2.50 vs 110.46 ± 3.85 in alveolar epithelial cells, with significant differences between groups （both P〈O.05） ; the levels of 13-eatenin expression were 86.97±4.87 vs 103.18± 3.77 in eytoplasm of bronchial epithelial cells, and 95.75 ± 3.91 vs 116.12 ± 5.76 in nuclei of bronchial epithelial cells, 106.24±4.57 vs 128.81±3.96 in cytoplasm of alveolar epithelial cells, and 125.44±4.89 vs 152.90：1：4.10 in nuclei of alveolar epithelial eells, with significant differences between groups （all P〈0.05）. Coneltmions Cigarette smoke may induce up-regulation of Wnt3a and [3-catenin proteins in rat lung tissue.

关 键 词：肺疾病 慢性阻塞性 WNT蛋白质类 Wnt-β-catenin信号途径 气道重构 香 烟烟雾 大鼠 

分 类 号：R563.9[医药卫生—呼吸系统]