人β防御素2在子宫内膜异位症和子宫腺肌症患者异位和在位内膜组织中的表达  

Human β defensin-2 expression of ectopic and eutopic endometrium in patients with endometriosis and adenomyosis

作　　者：李锦波[1] 陈淑琴[1] 姜红叶[1] 牛刚[1] 

机构地区：[1]中山大学附属第一医院妇产科,广州510080

出　　处：《中华生物医学工程杂志》2012年第1期25-30,共6页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金（81070472）

摘　　要：目的检测人β防御素2（hBD-2）在正常子宫内膜、子宫内膜异位症（EMS）和子宫腺肌症（AM）患者的异位和在位内膜组织中的表达及其临床意义。方法选择2007年2月至2008年4月本院本科的EMS（卵巢内膜异位囊肿）、AM和非EMS（子宫肌瘤）患者各25例，分别取卵巢异位囊肿囊壁组织（EMS异位内膜组）、AM异位病灶组织（AM异位内膜组）和两组相应的在位子宫内膜组织（EMS在位内膜组和AM在位内膜组）以及非EMS患者的子宫内膜组织（对照组）。采用实时荧光定量PCR（RT—PCR）和原位杂交检测各组内膜组织中hBD-2和IL-1β、IL-6、TNF-α等的基因表达及其相关性。结果将各组的hBD-2、IL-1β、IL-6和TNF-α的基因表达水平进行比较，仅EMS异位内膜组显著高于EMS在位内膜组和对照组（M0．3684比0．0343、0．0034；M0．1011比0．0295、0．0080：M0．0040比0．0008、0．0005；M0．0026比0．0005、0．0002；均P〈0．05）），而EMS在位内膜组与对照组、AM异位内膜组与AM在位内膜组、AM在位内膜组与对照组之间差异均无统计学意义（P〉0．05）。EMS异位内膜组hBD-2的基因表达水平与该组的IL-1β和TNF-α的基因表达水平呈正相关（r=0．867，r=0．683，P〈0．05），与IL-6的基因表达水平无相关性（r=0．167，P〉0．05）。结论EMS异位内膜组织中hBD-2的高表达可能参与EMS的形成，可能与EMS患者体内IL-1β和TNF-α的上调有关。Objective To investigate the expression and clinical significance of human β defensin- 2 （hBD2） in eutopic and ectopic endometrium in patients with ovarian endometriosis （EMS） or adenomyosis （AM）. Methods Seventy - five females with ovarian ectopie EMS （n=25） , AM （n=25） and non - EMS （hysteromyoma, n=25 ） in the First Affiliated Hospital of Sun Yat-sen University between February 2007 and April 2008 were enrolled in the study. The ovarian ectopic cyst wall（ectopic EMS group）, AM ectopic tissue （eetopic AM group ）, EMS eutopic tissue （eutopic EMS group ）, AM eutopic tissue （eutopic AM group ） and non-EMS endometrium （control group ） were obtained for quantitative analysis of hBD-2, IL-1 [3, IL-6 and TNF-ct expression as well as their correlations using real-time PCR （RT-PCR） and in situ hybridization. Results Higher expression levels of HBD-2, IL-1β, IL-6 and TNF-α genes were detected in ectopic EMS group as compared with those in eutopic EMS group and control group（M 0.3684 vs 0.0343 and 0.0034, M 0.1011 vs 0.0295 and 0.0080, M0.0040 vs 0.0008 and 0.0005, M 0.0026 vs 0.0005 and 0.0002, all P〈0.05 ）. In contrast, no significant difference（P〉0.05） was found in between-group comparison of eutopie EMS groupcontrol group, ectopic AM group-eutopic AM group, and eutopic AM group-control group. In ectopic EMS group, hBD-2 expression was positively correlated with IL-115 and TNF-α expression levels （r=0.867 and 0.683, respectively, both P〈O.05）, but was not correlated with IL-6 expression level（r=O.167, P〉O.05）. Conclusion High hBD-2 expression in EMS ectopic endometrium may play a role in the formation of EMS and may be associated with up-regulation of IL-1β and TNF-αx in vivo.

关 键 词：防御素 子宫内膜异位症 卵巢 子宫腺肌症 基因表达 

分 类 号：R711.71[医药卫生—妇产科学]