检测鼠疫耶尔森菌LcrV抗体的上转换发光技术免疫层析试纸条的制备及应用  

Preparation and application of up-converting phosphor technology-based immunochromatography test strip for detection of Yesina pestis LcrV antibody

作　　者：洪文艳[1] 王浩然[1] 王旺[1] 郭兆彪[1] 周蕾[1] 杨瑞馥[1] 

机构地区：[1]军事医学科学院微生物流行病研究所分析微生物室,北京100007

出　　处：《中华生物医学工程杂志》2012年第1期54-57,共4页Chinese Journal of Biomedical Engineering

基　　金：基金项目：国家科技支撑计划项目（2009BAK43831）

摘　　要：目的本研究旨在制备一种用于检测鼠疫耶尔森菌LcrV抗体的上转换发光技术（UPT）免疫层析试纸条。方法通过在试纸条分析膜检测带处固定抗原，质控带处固定抗体，结合垫位置半固定将上转换发光（UCP）颗粒标记的抗原，制备出了双抗原夹心模式的LcrV抗体检测UPT免疫层析试纸条。对制备成型后的试纸条进行了线性和灵敏度、准确性、及热稳定性4个方面的性能评价。最后用此试纸条检测了40份自然感染鼠疫的兔和人血清标本，并将结果与ELISA检测结果进行了比较。结果试纸灵敏度达0．97mg／L，对所测17份猴血清标本的检测准确度为100％，在37℃的稳定存放期为10d。试纸对40份实际血清标本的检测结果与ELISA检测结果一致。结论试纸有良好的灵敏度和线性检测能力。Objective To prepare an up- converting phosphor technology （UPT）- based immunochromatography test strip for detection of LcrV antibody in Yesina pestis （ Y. pestis）. Methods UPT-based chromatography test strips in double-antigen sandwich mode for detection of LcrV antigen were prepared through immobilizing the antigen at detection band and the antibody at quality control band as well as semifixing upconverting phosphor（UCP） particle-tagged antigen at the conjugation pad. Assessment of the linearity, sensitivity, accuracy and thermal stability was performed on well-prepared test strips. Finally, 40 respective copies of rabbit and human serum samples in those who were spontaneously infected by Y. pestis were detected using the strips, with the results being compared with those of ELISA. Results The sensitivity of the strips was 0.97 mg/L. The accuracy rate was 100% in 17 copies of monkey serum samples. The strips were capable of enduring a 10-day storage period in 37 ℃. The test results in 40 samples were identical with those using ELISA. Conclusion The strips have fine linearity, with good sensitivity.

关 键 词：鼠疫 色谱法 琼脂糖 抗体 上转换发光技术 

分 类 号：R446.6[医药卫生—诊断学]