五种鳞翅目害虫中抗药性相关基因的转录组学分析  

Application of transcriptome in insect resistance research

作　　者：刘莹[1] 王娜[1] 张赞[1] 李飞[1] 

机构地区：[1]南京农业大学植物保护学院,南京210095

出　　处：《应用昆虫学报》2012年第2期317-323,共7页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金(31171843)

摘　　要：随着测序技术的发展,昆虫转录组数据不断积累,在昆虫学研究中的应用也越来越广泛。在害虫抗药性的研究中,转录组数据分析也是重要的最新研究手段。本文通过对5种鳞翅目害虫转录组的生物信息学分析,鉴定出13种与抗药性相关的基因,发现细胞色素P450基因序列数量最多,共887条。另外,谷胱甘肽S-转移酶、鱼尼丁受体、氨肽酶N、糖基转移酶相关的基因序列多于100条。同时,对这5种鳞翅目昆虫中部分Bt受体相关的基因做了多序列比对和进化分析。分析认为,从多物种、多基因的角度提出对农药抗性的系统性研究,是害虫抗性研究的发展趋势。With the development of sequencing technology,the number of known insect transcriptome sequences has increased and transcriptome data has become more useful in entomology,including research on insect resistance.Here,we describe thirteen types of gene related to insecticide resistance in five Lepidopteran insects.In total,887 P450 gene sequences have been found.The numbers of Glutathione S-transferase,ryanodine,aminopeptidase N and glycosyltransferase genes alone exceed 100.Multiple sequence alignment and evolutionary analysis of Bt receptor genes has been conducted.We suggest that systematic analysis of multiple genes in single,or multiple,species will become possible in resistance research and that this will be a promising direction for future research.

关 键 词：转录组 抗药性基因 Bt受体 系统分析 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]