二斑叶螨对七种杀螨剂的抗药性测定及其机理研究  

The study on resistance and its mechanism of Tetranychus urticae to several common insecticides

作　　者：刘庆娟[1,2] 刘永杰[1] 于毅[2] 周仙红[2] 马惠 

机构地区：[1]山东农业大学,泰安271018 [2]山东省农业科学院山东省植物病毒学重点实验室,济南250100 [3]山东省棉花研究中心,济南250100

出　　处：《应用昆虫学报》2012年第2期376-381,共6页Chinese Journal of Applied Entomology

基　　金：公益性行业(农业)科研专项"农药高效安全科学施用技术"(200903033)

摘　　要：室内测定了相对敏感种群(S)和抗性种群(R)对常用7种杀螨剂的敏感性,并测定了羧酸酯酶、谷胱甘肽S-转移酶和乙酰胆碱酯酶3种酶的比活力。结果表明:二斑叶螨Tetranychus urticae Koch R种群已对甲氰菊酯和哒螨灵产生了抗性,抗性倍数分别为5.45和105.47。其中,甲氰菊酯对雌成螨的毒力最低(>3000mg/L),已远远超过田间推荐剂量,不宜继续使用。酶活测定结果表明:谷胱甘肽S-转移酶解毒活性的提高是二斑叶螨对甲氰菊酯产生抗性的原因之一;二斑叶螨对哒螨灵抗性的增强可能与羧酸酯酶有关。The frequent use of pesticides to control the two-spotted spider mite Tetranychus urticae Koch has accelerated the development of resistance in this mite.We evaluated the relative susceptibility of susceptible （S） and resistant （R） strains of T.urticae to several common acaricides.The results indicate that the R strain was 5.45 more resistant to fenpropathrin,and 105.47 times more resistant to pyridaben,than the S strain.Fenpropathrin had the lowest toxicity to female mites.The resistance of T.urticae is closely related to the activities of detoxicant enzymes.Measurement of the CarE,AChE and GST activities of the two strains indicates that pyridaben resistance can be attributed to increased carboxylesterase activity,and that the main cause of resistance to fenpropathrin is increased glutathione S-transferase activity.

关 键 词：二斑叶螨 甲氰菊酯 哒螨灵 解毒酶 

分 类 号：S433.7[农业科学—农业昆虫与害虫防治]