一种改进的粉虱成虫生物测定方法  

An improved bioassay method for adults of whiteflies

作　　者：田玉安[1] 杨茜茹[1] 梁沛[1] 高希武[1] 

机构地区：[1]中国农业大学昆虫学系,北京100193

出　　处：《应用昆虫学报》2012年第2期556-561,共6页Chinese Journal of Applied Entomology

基　　金：国家重点基础研究发展计划项目(2009CB119200);公益性行(农业)科研专项(201203038)

摘　　要：生物测定是检测害虫抗药性的一项重要技术。利用100mL插口圆底聚丙烯离心管对现在应用较多的粉虱成虫生物测定方法——琼脂保湿浸叶法进行了改进。改进后的方法不影响粉虱成虫的持续取食,具有操作简单、结果重复性好及无需对成虫麻醉等优点。同时发现茄子叶片对于B型烟粉虱Bemisia tabaci(Gennadius)B-biotype和温室白粉虱Trialeurodes vaporariorum(Westwood)成虫均是一种非常适合的生测材料。利用该方法分3次独立测定了烯啶虫胺对B型烟粉虱和温室白粉虱混合日龄成虫的毒力,结果具有很好的重复性。Bioassay is a very important technique for insecticide resistance detection.An improved bioassay method for adults of Bemisia tabaci （Gennadius）and Trialeurodes vaporariorum （Westwood）was reported in present paper.This method primarily composed of a cylinder tube,made from a centrifuge tube （38 mm in diameter and 107 mm in length） with bottom cut off and replaced with a piece of black cotton cloth to do the test.The nitenpyram treated eggplant-leaf disc,which was proved here to be a good material for the bioassay of adult whiteflies,was laid abaxial side down on a bed of agar gel inside the lid of the tube.The improved bioassay method described here offered a dependable,accurate,and relatively simple means to explore dose-response relationships in evaluating susceptibility of adult whiteflies to different varieties of insecticides.

关 键 词：B型烟粉虱 温室白粉虱 抗药性 生物测定 烯啶虫胺 

分 类 号：S433[农业科学—农业昆虫与害虫防治]