不同应激方式引发的抑郁大鼠行为特征比较  

The difference among the depression-like behaviours induced by three widely-used stress models in rats

作　　者：张丽[1] 朱熊兆[1] 白玫[1] 张逸[1] 张晟[1] 薛亮[1] 周晟[1] 蚁金瑶[1] 

机构地区：[1]中南大学湘雅二医院医学心理学研究所,长沙410011

出　　处：《中华行为医学与脑科学杂志》2012年第3期212-215,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（81071097,30670710）

摘　　要：目的了解经历不同类型的应激后大鼠抑郁样行为的差异。方法将新生的SD大鼠随机分为母爱剥夺应激组（27只）、慢性温和应激组（29只）、双重应激组（31只）和正常对照组（30只）。母爱剥夺组大鼠于出生后即接受母爱剥夺应激，慢性温和应激组大鼠在成年后接受慢性温和应激，双重应激组大鼠先接受母爱剥夺应激，成年后再接受慢性温和应激，对照组不接受任何实验处理。采用强迫游泳实验和糖水偏爱实验作为评估大鼠抑郁样行为的指标。结果在强迫游泳实验中，母爱剥夺组[（119．30±65．56）s]、慢性温和应激组[（145．00±80．24）S]及双重应激组[（170．03±61．75）S]大鼠静止漂浮时间长于对照组[（81．14±52．40）s，F=11．53，P〈0．01]，其中双重应激组长于母爱剥夺应激组（P〈0．01），慢性应激组和双重应激组、母爱剥夺组大鼠差异无统计学意义（均P〉0．01）；在糖水偏爱实验中，慢性温和应激组[（0．43±0．28）]、双重应激组[（0．50±0．26）]和对照组[（0．54±0．28）]大鼠的糖水偏爱率差异有统计学意义（F=4．33，P〉0．01），母爱剥夺组[（0．32±0．22）]的糖水偏爱率低于对照组和双重应激组（均P〈0．05）；雌、雄大鼠的静止漂浮时间和糖水偏爱率在不同应激方式下的差异无统计学意义（均P〉0．05）。结论3种应激方式均可导致大鼠表现出某种抑郁样行为。不同应激方式所致的抑郁样行为在不同性别的大鼠间无差异。Objective In order to study the difference of depression-like behaviours among three widely- used stress models in rats. Methods The new-born Spragne-Dawley rats were randomly divided into maternal dep- rivation（MD） group（n=27） ,chronic mild stress（CMS） group （n=29） ,dual stress（DS） group （n=31） and control （C） group （ n = 30） on postnatal day 1. MD rats only received maternal deprivation. CMS rats only received chronic mild stress when l0 weeks old. DS rats received both maternal deprivation and chronic mild stress. Control rats received no experimental handling. Behavior tests including forced swimming test and sucrose consumption which were carried out to evaluate rats＇depression-like behaviors in the thirteenth weeks. The extend time of float- ing and sucrose preference ratio should be recorded in the forced swimming test and sucrose consumption test respectively to reflect the behavior of helplessness and anhedonia of rats. Results In the forced swimming test, the extend floating time of MD group（ 119.30 ± 65.56 ） s, CMS group （ 145.00 ± 80.24） s and DS group （ 170.03 ±61.75 ） s were longer than the control group （ 81.14± 52.40） s （F = 11.53, P 〈 0.01 ）. In the sucrose consumption test, the MD group （0.32 ± 0.22） had a low sucrose consumption. The comparison of the MD group and CMS group （ 0.43 ±0.28 ） to the control group （0.54 ± 0.28 ） had significant differences （F = 4.33, P 〈 0.01 ）. In these four groups, no sexual difference was found in the forced swimming test and sucrose consumption test （ all P 〉 0.05 ）. Conclusion The results suggest that MD, CMS and DS may induce some kind of depression-like behaviors in rodents such as anhedonia or the behavior of helplessness and the depression-like behaviors induced by different stresses are similar in male and female individuals.

关 键 词：应激 抑郁样行为 强迫游泳实验 糖水偏爱实验 性别差异 

分 类 号：R395.6[哲学宗教—心理学]