不对称大环双核Ni(Ⅱ)配合物的DNA切割性能研究(英文)  

Studies on an Unsymmetrical Macrocyclic Dinuclear Ni(Ⅱ) Complex Interaction with DNA

作　　者：丁超[1] 陈云峰[1] 刘明[1] 宋会婷[1] 周红[1] 潘志权[1] 

机构地区：[1]武汉工程大学绿色化工过程教育部重点实验室,武汉430073

出　　处：《无机化学学报》2012年第4期801-808,共8页Chinese Journal of Inorganic Chemistry

基　　金：国家科学自然基金(No.20971102);湖北省教育厅中青年人才项目(No.Q20111507)资助

摘　　要：本文利用模板导向的方式合成了一种含有不对称侧链的大环双核金属Ni(Ⅱ)配合物(大环配体由3-溴甲基-5-甲基水杨醛,乙二胺,N,N-二(氨丙基)-2呋喃甲胺分步合成得到),其结构通过红外光谱、元素分析、X-射线单晶衍射进行了表征。利用紫外光谱、分子荧光和粘度试验对配合物与DNA的相互作用进行了研究,结果表明配合物与CT-DNA的结合常数K=4.8×104mol-1.L荧光淬灭常数Ksv=7.12×103mol-1.L;同时机理研究表明配合物与CT-DNA结合方式为插入模式。An unsymmetrical side chains macrocyclic dinuclear Ni（ll） complex has been obtained by templatedirected synthesis （the maeroeyelic ligand was synthesized by 3-bromomethyl-2-hydroxy-5-methylbenzaldehyde, ethane-l,2-diamine, and Nl-（3-aminopropyl）-Nl-（furan-2-ylmethyl）propane-l,3-diamine）, the structure has been characterized by IR spectrum, elemental analysis and X-ray crystallography determination. The interactions of the complex with DNA have been measured by UV spectroscopy, fluorescence spectroscopy and viscosity experiments. Absorption spectroscopic investigation reveals of intercalative binding of the complex with DNA with a binding constant of 4.8x104 mo1-1.L. Fluorescence spectroscopy shows that the complex displaces EB and bind to DNA with a quenching constant of 7.12x103 mol-1L. The appearance of both increased CD bands nearing 371 nm gives evidence for effective eomplex-DNA binding. The agarose gel electrophoresis studies show that the complex displays effective DNA cleavage activity in the absence of any external agents. CCDC： 835716.

关 键 词：同双核配合物 大环配合物 结合常数 粘度 DNA切割 

分 类 号：O614.813[理学—无机化学]