绿色荧光蛋白转基因神经干细胞脑出血大鼠脑内移植后血管生成与神经形态学的改变  

Vasculogenesis and neural norphology change after brain transplantation of neural stem cells over- expressing green fluorescent protein to the rats with intracerebral hemorrhage

作　　者：毛进鹏[1] 诸葛启钏[1] 刘乐平[2] 

机构地区：[1]温州医学院附属第一医院神经外科,325000 [2]温州医学院外科实验室

出　　处：《中华实验外科杂志》2012年第4期690-692,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30870878）

摘　　要：目的观察绿色荧光蛋白转基因神经干细胞（NSCs／GFP）脑出血大鼠脑内移植后血管生成与神经形态学改变。方法分离、培养及纯化NSCs／GFP，并进行体外诱导分化。注射动脉血制作大鼠脑出血模型，3d后将NSCs／GFP注入血肿同侧尾状核，观察NSCs／GFP在脑内分化情况以及大鼠不同时间点行为学的变化。结果成功分离、培养及鉴定NSCs／GFP，体外诱导分化为内皮细胞和平滑肌细胞；移植入脑出血大鼠脑内后，能促进其肢体功能恢复（P〈0．05），且能分化为神经元、星型胶质细胞、内皮细胞及平滑肌细胞。结论NSCs／GFP移植入脑出血大鼠脑内能改善其运动功能，并有生成血管与神经的能力。Objective To explore the vasculogenesis and neural norphology change after brain transplantation of neural stem cells overexpressing green fluorescent protein （NSCs/GFP） to the rats with intracerebral hemorrhage. Methods NSCs/GFP were separated, cultured and purified. NSCs/GFP were induced to differentiate into endothelial cells and vascular smooth muscle cells. The rat intracerebral hem-orrhage models were made by injecting arterial blood and NSCs/GFP were transplanted to ipsilateral caudate necleus three days later. The differentiation of NSCs/GFP transplanted to animals was observed. The chan- ges of behaviors at different time points were observed. Results NSCs/GFP were successfully separated, cultured, identified and induced to differentiate into endothelial cells and smooth muscle cells in vitro. NSCs/GFP could differentiate into neurons, astrocytes, endothelial cells and smooth muscle cells around the hematoma. Neural functional recovery was much better in the group of NSCs/GFP transplantation than in the contral group （P 〈 0.05）. Conclusion Transplantation of NSCs/GFP promotes functional recovery in rats with intracerebral hemorrhage. NSCs/GFP have the capacity of neurogenesis and vasculogenesis.

关 键 词：神经干细胞 脑出血 移植 血管生成 

分 类 号：R651.1[医药卫生—外科学]