糖尿病大鼠膀胱肾上腺素能β3受体的改变  

Changes of β3-adrenergic receptor in the bladder of rats with diabetes

作　　者：蒋玉清[1] 赵万里[3] 王秀丽[2] 郭跃先[1] 

机构地区：[1]河北医科大学第三医院泌尿外科,石家庄050051 [2]河北医科大学第三医院麻醉科,石家庄050051 [3]河北沧州市中心医院泌尿外科

出　　处：《中华实验外科杂志》2012年第4期699-702,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30772082）;河北省自然科学基金资助项目（C2008000931）

摘　　要：目的观察糖尿病大鼠膀胱中肾上腺素能B3受体（β3-AR）的表达在糖尿病膀胱病变发生发展中的作用。方法将80只大鼠按随机化原则分为糖尿病模型组（n=40）及正常对照组（n=40）。应用链脲佐菌素60mg／kg腹腔注射，诱导形成I型糖尿病大鼠模型，分别在发病第2、4、6、8、10周时处死取全膀胱，称重，取材制作光镜病理标本及提取蛋白，采用蛋白印迹法检测各组大鼠膀胱逼尿肌细胞肾上腺素能β3受体蛋白的表达。结果糖尿病模型组大鼠体质量组增长缓慢，膀胱湿重增加（P〈0．05）。糖尿病组膀胱逼尿肌细胞肥大，变性，排列紊乱松散，胶原及纤维组织明显增生，出现空泡；晚期逼尿肌细胞萎缩，仅见大量的胶原及弹性纤维成分。糖尿病大鼠膀胱逼尿肌细胞肾上腺素能B3受体蛋白表达高于正常对照组（P〈0．05）；2周：（0．73±0．09）比（0．35±0．04），4周：（0．81±0．13）比（0．32±0．06），6周：（0．72±0．05）比（0．33±0．04），8周：（0．55±0．07）比（0．33±0．06），10周：（0．52±0．07）比（0．33±0．05）（P〈0．05）。结论各时间段的糖尿病组膀胱逼尿肌均出现损害，各时间段的糖尿病组膀胱逼尿肌细胞中肾上腺素能β3受体含量均增加，肾上腺素能B3受体在糖尿病膀胱病变的发生发展中发挥萤要作用．Objective To investigate the expression of β3-adrenergic receptor （β3-AR） and de- trusor injury in the bladder of rats with diabetic mellitus. Methods Eighty female Wistar rats were ran- domly divided into the diabetic model group （ n = 40） and the normal control group （ n = 40）. The rat type 1 diabetes mellitus model was induced by peritoneal injection of the streptozotocin. The experiments were carried out at corresponding time points after the model was successfully set up, and the blood glucose （BG） levels were measured to exclude those rats with BG≤16. 7 mmol/L. The bladders were completely cut down and the bladder wet weight was measured. After hematoxylin and eosin （HE） staining, the mor-phological changes of the bladder detrusor in diabetic rats were observed under the light microscope. The expression of β3-AR protein was detected by using Western blotting. Results Routine pathologic examinations of the bladder detrusor smooth muscle ： Under the light microscope, detrusor structure in every group was observed. In diabetic model group, transitional epithelium cell arrangement was not intact. Membrana propria was hyperemia and dropsy and size of decompensated detrusor muscle cell was large and the degeneration appeared, the shape was diverse and interstitial collagen component increased. After that, Muscle fiber was arranged disorder and out of order, cross of muscle fiber was full and space between the muscle fiber was enlarged obviously. The collagen and fiber structure was obviously proliferated and a lot of collagen could be seen. Finally detrusor cell atrophied, reduced or even disappeared, and only a lot of collagen and elastic fiber components could be seen. The change in β3-AR protein of the bladder detrusor smooth muscle： The result of Western blotting demonstrated that the amount of β3-AR protein in diabetic rat bladder was increased than control bladder [ 2 w ： （0. 73±0.09 ） vs （0. 35 ±0. 04 ） ,4 w ： （0. 81±0. 13 ） vs （0. 32±0.06） ,6 w：（0

关 键 词：肾上腺素能β3受体 逼尿肌 糖尿病 膀胱 

分 类 号：R587.1[医药卫生—内分泌]