异种脱蛋白骨复合成骨细胞修复兔桡骨骨缺损的研究  

Repair of rabbit radial defect by using xenogenic deproteinized bone scaffold

作　　者：陈长青[1] 程高建[2] 练克俭[1] 黄立羡[1] 丁真奇[1] 康两奇[1] 

机构地区：[1]厦门大学附属东南医院骨科医院解放军第175医院全军创伤骨科中心,漳州363000 [2]西安医学院西安红十字会医院骨科

出　　处：《中华实验外科杂志》2012年第4期732-734,共3页Chinese Journal of Experimental Surgery

基　　金：南京军区“十一五”重点计划课题（06229）;福建省青年科技人才创新项目（2004F3146）

摘　　要：目的观察异种脱蛋白骨（DPB）复合皮质骨来源成骨细胞修复兔桡骨临界骨缺损的效果。方法36只新西兰大白兔的桡骨建立1．5cm临界骨缺损模型，随机分为A、B、C3组，分别植入：A组，异种脱蛋白骨与成骨细胞复合物；B组，自体髂骨；C组，异种脱蛋白骨。术后12周检测如下：X线观察、骨密度测量、标本大体观察、生物力学测试、组织学观察。结果x线显示：A、B两组均可见植入体与桡骨融合，骨折线消失，C组成骨量少，骨折线仍存在。骨密度测量：A、B两组差异无统计学意义（F=2．388，P〉0．05）；A、C两组的差异有统计学意义（F=10．869，P〈0．05）。标本大体观察：A、B两组骨折线消失；C组骨折线存在。生物力学测试：A、B组差异无统计学意义（F=1．353，P〉0．05）；A、C两组差异有统计学意义（F=4．395，P〈0．05）。组织学观察：A组大量新生骨，B组被周围骨爬行替代，C组少量骨形成。结论异种脱蛋白骨复合成骨细胞可以高效修复兔桡骨临界骨缺损。Objective To evaluate the efficiency of xenogenic deproteinized bone （DPB） based on cortical bone-derived osteoblasts as an alternative in repair of critical bone defect. Methods A total of 36 Newzealand rabbits were randomly divided into groups A, B and C. Critical bone defect with a length of 1.5 cm was created in one side of the radius. The defect was filled with a DPB scaffold seeded with osteo- blasts （group A）, autograft （group B） and DPB scaffold （group C ）. All specimens were selected for X-ray examination, bone mineral density （BMD） measurements, gross samples observation, biomechanical test and histological observation at 12th week postoperation. Results X-ray examination displayed that the radial defect which was still obvious in group C disappeared in groups A and B. BMD measurements showed that there was no significant differenee in BMD between groups A and B, but that was higher in group C （P 〈0. 05 ）. Gross samples observation revealed that the radial defect which was still obvious in group C disappeared in groups A and B. Biomechanical testing demonstrated that the maximum bend load in groups A and B was significantly higher than in group C （ P 〈 0. 05 ）. Histologically, there was abundant of newly bone formation in group A, the medullary cavity recanalized in group B, and a small amount of newly bone formation in group C. Conclusion Radial eritieal bone defect could be repaired efficiently by xenogenic DPB based on cortical bone-derived osteoblasts.

关 键 词：成骨细胞 脱蛋白骨 骨缺损 

分 类 号：R687.3[医药卫生—骨科学]