A型肉毒杆菌毒素对佐剂关节炎大鼠的镇痛作用  

The analgesic effects of intra-articular botulinum toxin type A and mechanisms in chronic knee arthritis pain model

作　　者：茹靖涛[1] 曹靖[3] 秦涛[2] 

机构地区：[1]河南省人民医院急诊创伤外科,郑州450003 [2]河南省人民医院器官移植中心,郑州450003 [3]郑州大学基础医学院

出　　处：《中华实验外科杂志》2012年第4期735-737,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（31140078）

摘　　要：目的观察关节腔内注射A型肉毒杆菌毒素（BoNT／A）在佐剂关节炎模型中的镇痛效果及疼痛相关物质在脊神经节及脊髓的变化。方法sD大鼠右后足跖部皮下注射完全弗氏佐剂（CFA）0．1ml建立类风湿性关节炎的动物模型（AA）。关节炎形成后随机分为3组：BoNT组（n=10）：关节腔内注射Botox／A0．02IU／5μl；NS组（n=10）：关节腔内注射生理盐水5μl；Sham组（n=10）：非关节炎模型组。5d后检测疼痛行为学、力量评估；免疫组织化学检测脊神经节钠离子通道1．8（Nav1．8）、脊髓N-甲基-D-天冬氨酸受体2B亚基（NR2B）的表达。结果肉眼步态分析显示大鼠关节炎引起的严重的步态异常，BoNT治疗组改善了40％（P〈0．05）；治疗组大鼠右足热痛阈升高（P〈0．05）；脊神经节Navl．8高表达；脊髓NR2B表达降低。结论通过关节腔内注射Botox／A可以抑制疼痛信号的传导，减轻关节痛。Objective To estimate the analgesic effect of intra-articular injection of botulinum toxin type A （BoNT/A） in adjuvant arthritis model and to explore the related factors associated with arthritis pain. Methods We established murime model of chronic knee arthritis pain by injection of complete Freund＇s adjuvant （CFA） O. 1 ml into the right paw of Sprague Dawley rats. Following induction of arthritis, BoNT/A group （n = 10） received intra-articular BoNT/A0. 02 IU/5 μL; NS group （n = 10） was treated with intra-articular 5 μL saline injections. In Sham group （n = 10）, no artiritis model was established. Pain behavior testing was performed prior to arthritis, after induction of arthritis, and following treatments. Pain behavior measures included analysis of gait impairment and thermal pain threshold. Strength was measured as ability to cling. Pain related factors Navl. 8 and NR2B were detected by using immunohisto-chemistry. Results Gait analysis showed that significant impairment of gait in arthritic mice wast improved by 40% （P 〈 0.05） after intra-articular BoNT/A. Thermal hyperalgesia threshold of treatment group was decreased after intra-articular BoNT/A （P 〈 0. 05 ）. The expression of Navl. 8 in ganglia and NR2B in the spinal cord was also decreased significantly. But no systemic effects or limb weakness was noted. Conclusion Intra-articular injection of BoNT/A can reduce the pain of adjuvant arthritis by inhibit the transmission of pain signals.

关 键 词：肉毒杆菌毒素 关节炎 镇痛 

分 类 号：R684.3[医药卫生—骨科学]