基于新型还原剂羊毛角蛋白的高效溶解  

The Efficient Dissolution of Wool Based on New Reductant

作　　者：王江波[1] 刘建勇[1,2] 王洁[1] 张玉荣[1] 

机构地区：[1]天津工业大学纺织学院,天津300160 [2]天津工业大学先进纺织复合材料教育部重点实验室,天津300160

出　　处：《材料导报》2012年第6期7-9,13,共4页Materials Reports

基　　金：天津市科委支撑计划项目(10ZCKFSH00800)

摘　　要：羊毛角蛋白的溶解已经有几十年的历史,但一直没有发现较好的溶解方法。首次将生化用新型精细二硫键还原剂TCEP(三羧乙基膦)应用于羊毛角蛋白的溶解。TCEP对二硫键有很强的选择还原性,与亚硫酸氢钠联合使用,与以往的羊毛溶解方法相比有很大优势,使羊毛的溶解效率大大提高,溶解时间为5h时,溶解率达96.3%。通过红外测试推断,少量的不溶物为胱氨酸含量较高的高或超高硫蛋白。角蛋白浓度达10%,所制得的角蛋白溶液分子量在3500D以上的达80%,8000D以上的达72%。通过凝胶电泳分析,得到角蛋白的主体分子量为14000～20000D。The dissolution of the wool keratin has a history of several decades, but has not found an effective dissolution method. A novel biochemical disulfide reducing agent-TCEP（Tris（2-carboxyethyl） phosphine） was applied to the dissolution of wool keratin for the first time. TCEP has a strong selection of disulfide reduction, and joint use of sodium bisulfite, the dissolution of the previous methods have great advantages in wool. The efficiency of dissolution was greatly improved; dissolution time was 5h, dissolution rate was 96.3%. Through FT-IR, the small amount of in- soluble matter was high or ultra high sulfur protein which has a high content of eystine maybe. Keratin concentration was 10%, the molecular yield of keratin more than 3500D and more than 8000D were 80% and 72%. By SDS-PAGE, the body molecular of the keratin was 14000-20000D.

关 键 词：羊毛溶解三羧乙基膦亚硫酸氢钠透析凝胶电泳红外 

分 类 号：TQ340.61[化学工程—化纤工业]