单肺通气大鼠肺泡表面活性蛋白表达的研究  

Changes in pulmonary surfactant proteins during one lung ventilation in rats

作　　者：易娟[1] 黄冰[2] 黎阳[2] 阮林[2] 彭丹晖[2] 林飞[2] 顾永辉[2] 黄宇[2] 陈锡华[1] 

机构地区：[1]广西医科大学研究生学院 [2]广西医科大学附属肿瘤医院麻醉科,南宁530021

出　　处：《中国癌症防治杂志》2012年第1期42-45,共4页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：广西自然科学基金资助项目(桂科自NO0832232)

摘　　要：目的研究单肺通气(OLV)大鼠肺组织肺泡表面活性蛋白A(SP-A)和肺泡表面活性蛋白B(SP-B)表达的变化。方法 42只SD大鼠随机分成单肺通气组(A组)、双肺通气对照组(B组)和空白对照组(C组)。A组和B组根据不同的通气时间分成亚组A1、A2、A3组和B1、B2、B3组,将气管导管过深插管至右肺建立右肺OLV模型。分别取各组左右两侧肺组织,光镜下观察其肺泡结构及肺间质的病理变化,免疫印迹法检测肺组织中SP-A和SP-B蛋白水平。结果与C组相比较,随着通气时间的延长,A组和B组肺组织逐渐表现出肺泡充血水肿、肺间质逐渐增厚,且在A组非通气侧这些病理变化更为严重。与C组比较,A组各亚组SP-A和SP-B蛋白表达均减少(P<0.05),且随着OLV时间延长,蛋白表达减少更加明显(P<0.05);与B组各亚组对应侧肺比较,A组中各亚组左侧肺SP-A和SP-B蛋白表达均减少(P<0.05),右侧肺SP-A蛋白表达差异无统计学意义(P>0.05),右侧肺SP-B蛋白表达在A1、A2组差异无统计学意义(P>0.05),而在A3组则比B3组蛋白表达减少(P<0.05);A组各亚组组内两侧肺比较,SP-A和SP-B蛋白在A组各亚组左侧肺均比右侧肺蛋白表达减少(P<0.05)。结论 OLV可致SP-A和SP-B表达下调,且非通气侧比相同时间的双肺通气肺组织下调更明显,这可能是OLV所致肺损伤更加严重的重要因素。Objective To study changes in pulmonary surfactant proteins A（SP-A） and B（SP-B） in rat lung tissue after one lung ventilation.Methods Sprague-Dawley rats（n=42） were randomly divided into three groups：one lung ventilation group（group A）,two lung ventilation group（group B）,and control group（group C）.Groups A and B were further subdivided depending on the ventilation time（A1,A2,A3;B1,B2,B3）.An artificial endotracheal tube was pushed into the right main bronchus in order to perform one lung ventilation.Pathological changes in the lung were examined by light microscopy,and the expression of SP-A and SP-B in lung tissues was detected by Western blot.Result In contrast to group C,groups A and B showed intra-alveolar exudates,hemorrhaging and inter-stitial edema that was proportional to ventilation duration.The left（unventilated） lung showed more serious damage than the right lung in group A.Levels of SP-A and SP-B were significantly lower in group A than in group C（P0.05）,and the levels in group A de-creased with increasing OLV duration（P0.05）.In the left lung,levels of SP-A and SP-B were significantly lower in A1,A2,and A3 than in B1,B2,and B3,respectively（P0.05）.In the right lung,there were no significant differences between groups A and B in the expression of SP-A（P0.05）,nor were there significant differences between A1 and B1 or A2 and B2 in the expression of SP-B（P0.05）.However,the levels of SP-B were lower in A3 than in B3（P0.05）.The levels of SP-A and SP-B were lower in the left lung than in the right one in A1,A2,and A3（P0.05）.Conclusion OLV causes the levels of SP-A and SP-B in lung tissue to decrease be-low the levels observed with two lung ventilation of the same duration.This may be a primary cause of the more serious lung injury caused by OLV.

关 键 词：单肺通气 肺泡表面活性蛋白A 肺泡表面活性蛋白B 

分 类 号：R655[医药卫生—外科学]