补骨脂雌激素样作用实验研究  

Study on Phytoestrogenic Effect in Psoralea Colrylifolia

作　　者：李璘[1] 邱蓉丽[1] 乐巍[1] 鞠晓云[1] 范乃兵[1] 宋宏绣[1] 李寰舟[1] 

机构地区：[1]南京中医药大学,江苏南京210029

出　　处：《辽宁中医药大学学报》2012年第4期57-58,共2页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：江苏省中医药管理局项目(LB09013);南京中医药大学国家基金预研基金资助项目(XYY06)

摘　　要：目的:通过实验方法确定补骨脂是否具有雌激素样作用。方法:采用子宫增重实验、ELISA实验法,通过对幼龄小鼠子宫重理和血清中相关激素水平的影响,观测补骨脂是否具有雌激素样作用。结果:在子宫增重实验中,补骨脂明显升高动物子宫系数;在血清雌激素与睾酮含量水平测定实验中,补骨脂明显能升高小鼠的血清雌激素水平(P<0.01),同时,雄激素水平也有升高的趋势,但与正常组相比无明显差异。结论:补骨脂具有雌激素样作用,同时也影响了体内雄激素的水平。补骨脂可能通过提高下丘脑-垂体-性腺轴的敏感性,从而影响血清中雌性激素和雄性素,发挥双向调节的作用。To explore the phytoestrogenic effects of psoralea colrylifolia L., the Chinese medicine. Methods： 50 prefemale ICR mice were randomly divided into five main groups and after administered （ op ）for 7 days, blood was collected and serum was separated. At the same time, the uterine weight of mice in each group was weighted. The levels of E2 and T in serum were analyzed by ELISA assay. Results ： Psoralea colrylifolia L could increase the uterine weight in premice and also markedly enhance the level of E2 and T in serum. Conclusion ： Psoralea colrylifolia L show the estrogenic effects and it is highly recommended that the application of psoralea colrylifolia L on treating the kidneyyang Deficiency was relative to its phytoestrogenic effects.

关 键 词：补骨脂 雌激素 雄激素 ELISA 

分 类 号：R282.71[医药卫生—中药学]