不同强度脉冲超声波对围绝经期大鼠卵泡发育的影响  

Impact of pulsed ultrasound on the development of ovarian follicle in perimenopausal SD rats

作　　者：翟蓓[1] 方廖琼[1] 李成志[1] 林川[1] 

机构地区：[1]重庆医科大学生物医学工程学院,省部共建超声医学工程国家重点实验室,超声医学工程重庆市市级重点实验室,重庆400016

出　　处：《中国介入影像与治疗学》2012年第4期304-306,共3页Chinese Journal of Interventional Imaging and Therapy

基　　金：国家自然科学基金(31000435);国家重点基础研究发展计划(973计划)项目(2011CB707902)

摘　　要：目的观察不同强度脉冲超声波对围绝经期SD大鼠卵泡发育的影响。方法选择自然老化的围绝经期SD大鼠24只,随机分为0.5W/cm2组、0.8W/cm2组和对照组。对照组不予辐照,0.5 W/cm2组和0.8 W/cm2组每天予以相应强度的脉冲超声波(频率均为1.02MHz)辐照10min,10天后处死各组大鼠。采用化学发光法测定血清雌激素,HE染色观察各级卵泡发育情况和卵泡总数变化。结果与对照组相比,0.5W/cm2超声组和0.8W/cm2超声组的雌激素水平升高、窦前卵泡数增多,0.8W/cm2超声组的原始卵泡数减少(P均<0.05)。结论低强度脉冲超声波可以促进围绝经期SD大鼠的卵泡发育。Objective To evaluate the effects of pulsed ultrasound at different intensities on development of ovarian follicle in perimenopausal SD rats.Methods Twenty-four female SD rats during perimenopausal period were selected by unforced aging,and the rats were randomly divided into 0.5 W/cm^2 group,0.8 W/cm^2 group and control group.Rats in the control group was not given any irradiation,while rats in 0.5 W/cm^2 group and 0.8 W/cm^2 group were given 10 min of pulsed ultrasound daily for 10 days with 0.5 or 0.8 W/cm^2 intensity,respectively.All the rats were sacrificed on the 11th day.Serum estrogen was measured by chemiluminescence-immunoassay,and different levels and total follicles were calculated.Results The levels of estrogen were higher in the 0.5 W/cm^2 and 0.8 W/cm^2 groups than that of control group（both P0.05）.Comparing with control group,primordial follicle quantity decreased in 0.8 W/cm^2 group,and primary follicle quantity increased both in the 0.8 W/cm^2 group and 0.5 W/cm^2 group（all P0.05）.Conclusion Low intensity pulsed ultrasound can promote the development of ovarian follicle in perimenopausal SD rats.

关 键 词：超声检查 围绝经期 卵巢形态学 

分 类 号：R445.1[医药卫生—影像医学与核医学] R711.75[医药卫生—诊断学]